中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (2): 104-106.

• 论著 • 上一篇    下一篇

临床少见致病酵母的致病力和体外药物敏感性及电泳核型的初步研究

胡晓莉, 李少平, 王红, 刘维达   

  1. 中国医学科学院、南京中国协和医科大学皮肤病研究所, 南京 210042
  • 收稿日期:1999-04-09 发布日期:2000-04-15

Study on Pathogenicity,Susceptibility to Antifungal Drugs and Electrophoretic Karyotype of Uncommon Pathogenic Yeasts

HU Xiaoli, LI Shaoping, WANG Hong   

  1. Institute of Dermatology, Chinese Academy of Medcial Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1999-04-09 Published:2000-04-15

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摘要： 目的 比较10种临床少见酵母致病力的大小,体外对抗真菌药物敏感性的高低以及电泳核型的差异。方法 用经环磷酰胺和氢化可的松联合免疫抑制的小鼠为感染模型,分别用10种酵母攻击,然后观察其存活期,并计数重要脏器中酵母的CFU;用微量稀释法测定这些酵母对氟康唑和酮康唑的MIC值;用脉冲场凝胶电泳技术确定这些酵母的电泳核型。结果 不同酵母的致病力大小不同,但均低于白念珠菌;对两种抗真菌药物的敏感性,不同酵母之间有差异;各酵母的染色体的大小和数目的区别则反映了其基因型特征。结论 不同的临床少见酵母其致病力,对氟康唑和酮康唑的敏感性及基因型均有较大差异。这些资料对临床诊治这类酵母引起的条件性真菌感染可有较大帮助。

关键词: 酵母菌, 微生物敏感性试验, 基因, 真菌

Abstract: Objective To investigate the pathogenicity, susceptibility to antifungal drugs, and electrophoretic karyotype of clinically uncommon pathogenic yeasts. Methods The immunocompromised mice, induced by hydrocortisone and cyclophosphamide, were challenged by the yeast cells, then their survival periods were observed, and the CFU/mL of yeast cells in the important internal organs were counted. The minimal inhibitory concentrations(MICs) of the yeasts were determined with microdilution method. The genomic total chromosomes of these yeasts were separated by PFGE. Results The pathogenicity of 10 yeasts,namely, Torulopsis glabrata, Sporobolomyces salmonicolor,Saccharomyces cerevisiae,Cryptococcus laurentii, Debaryomyces hansenii,Torulopsis globular,Trichosporon beigelii, Klocckera lemom, Geotrichum candidum and Rhodotorula rubra,varied and all were less virulent than that of C.albicans. The susceptibility of the yeasts to two antifungal agents,fluconazole and ketoconazole, was different.The PFGE pattern showed that different yeasts had different electrophoretic karyotypes. The numbers of chromosomes were 12 for S. cerevisiae,6 for T.beigelii,9 for D.hansenii,8 for T.glabrata,4 for K.lemom,7 for R.rubra,5 for S.salmonicolor and 10 for C.laurentii. Conclusion The clinically uncommon yeasts tested yield different pathogenicity, antifungal susceptibility and genotypes. These data could be helpful for the clinical diagnosis and treatment of mycoses caused by these yeasts.

Key words: Yeasts, Microbial sensitivity tests, Genes, fungal