中华皮肤科杂志 ›› 2020, Vol. 53 ›› Issue (8): 616-622.doi: 10.35541/cjd.20191169

• 论著 • 上一篇    下一篇

特应性皮炎患者面部、上肢和背部皮损真菌群落多样性分析

陆茂1    冉玉平1    代亚玲1    欧美2    吴红梅2    罗媛元2   

  1. 1四川大学华西医院皮肤科，成都  610041；2成都医学院第一附属医院皮肤科  610500
    陆茂现在成都医学院第一附属医院皮肤科，成都  610500

  • 收稿日期:2019-12-20 修回日期:2020-05-05 发布日期:2020-07-31
  • 通讯作者: 冉玉平 E-mail:ranyuping@vip.sina.com
  • 基金资助:
    四川省教育厅科研计划项目（17ZA0127）

Diversity of fungal communities on lesions of the face, upper limbs and back in patients with atopic dermatitis

Lu Mao1, Ran Yuping1, Dai Yaling1, Ou Mei2, Wu Hongmei2, Luo Yuanyuan2   

  1. 1Department of Dermatology, West China Hospital, Sichuan University, Chengdu 610041, China; 2Department of Dermatology, The First Affiliated Hospital of Chengdu Medical College, Chengdu 610500, China
    Lu Mao is working on the Department of Dermatology, The First Affiliated Hospital of Chengdu Medical College, Chengdu 610500, China
  • Received:2019-12-20 Revised:2020-05-05 Published:2020-07-31
  • Contact: Ran Yuping E-mail:ranyuping@vip.sina.com
  • Supported by:
    Research Project of Sichuan Provincial Education Department（17ZA0127）

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摘要： 【摘要】 目的 探讨特应性皮炎（AD）患者面部、上肢和背部皮损真菌群落多样性和结构特征。方法 对2015年9 - 10月来自成都医学院第一附属医院皮肤科门诊的10例AD患者面部、上肢和背部皮损区和10例健康对照相应部位皮肤采样，提取样品DNA行MiSeq高通量测序，进行多样性指数分析、物种组成分析及主成分分析。两组间比较采用独立样本t检验，多组间均数比较采用单因素方差分析，两两组间多重比较采用LSD-t检验。结果 多样性指数分析显示，AD患者组面部、上肢、背部皮损区样本Shannon指数均显著高于健康对照组相应区域（t值分别为2.67、2.37、3.34，均P < 0.05）。物种组成分析显示，马拉色菌属在AD患者组面部、上肢、背部皮损区和健康对照组相应区域样本中均占主要地位，其中球形马拉色菌和限制马拉色菌丰度合计约为80%。AD患者组总样本念珠菌属、曲霉属丰度显著高于健康对照组（t值分别为3.515、2.137，均P < 0.05）。AD患者组与健康对照组面部各主要真菌属丰度差异均无统计学意义（均P > 0.05）；AD患者组上肢念珠菌属丰度显著高于健康对照组（t = 3.186，P < 0.05），背部曲霉属丰度显著高于健康对照组（t = 2.736，P < 0.05）。AD患者组、健康对照组面部、上肢、背部样本间主要真菌属丰度差异均无统计学意义（P > 0.05）。轻中重度AD患者组样本间各主要真菌属丰度比较，差异均无统计学意义（均P > 0.05）。主成分分析显示，AD患者组面部、上肢、背部皮损样本真菌群落未按病情严重程度聚类。结论 AD患者面部、上肢、背部皮损区真菌群落多样性明显高于健康对照相应区域，马拉色菌为AD患者上述部位皮损和健康对照相应区域的优势菌种，AD患者皮损区样本中真菌群落组成与疾病严重程度可能无相关性。

关键词: 皮炎, 特应性, 皮肤, 真菌, 高通量核苷酸序列分析, 生物多样性

Abstract: 【Abstract】 Objective To investigate the diversity and structural characteristics of fungal communities on lesions of the face, upper limbs and back in patients with atopic dermatitis （AD）. Methods Samples were collected from the lesions on the face, upper limbs and back of 10 AD patients, who visited the Department of Dermatology, the First Affiliated Hospital of Chengdu Medical College from September to October in 2015, and collected from the corresponding body sites of 10 healthy controls. DNA was extracted from the samples, and subjected to MiSeq high-throughput sequencing for diversity index analysis, species composition analysis and principal component analysis. Statistical analysis was carried out by using two-independent-sample t test for comparisons between two groups, one-way analysis of variance for comparisons among multiple groups, and least significant difference-t test for multiple comparisons. Results Diversity index analysis showed that Shannon index was significantly higher in the samples from the lesions on the face, upper limbs and back of the AD patient group than in those from corresponding body sites of the healthy control group （t = 2.67, 2.37, 3.34 respectively, all P < 0.05）. Species composition analysis showed that Malassezia was predominant in the skin samples from the face, upper limbs and back of the AD patient group and healthy control group, and the total abundance of Malassezia globosa and Malassezia restricta was about 80%. The abundance of Candida and Aspergillus in the total samples was significantly higher in the AD patient group than in the healthy control group （t = 3.515, 2.137 respectively, both P < 0.05）. There was no significant difference in the abundance of major fungal genera on the face between the AD patient group and healthy control group（all P > 0.05）; the abundance of Candida in the upper limbs was significantly higher in the AD patient group than in the healthy control group （t = 3.186, P < 0.05）, and the abundance of Aspergillus in the back was significantly higher in the AD patient group than in the healthy control group （t = 2.736, P < 0.05）. In either the AD patient group or the healthy control group, there was no significant difference in the abundance of major fungal genera among samples from the face, upper limbs and back （all P > 0.05）. Moreover, no significant difference in the abundance of major fungal genera was observed among the mild, moderate and severe AD patient groups （all P > 0.05）. Principal component analysis showed that fungal communities in the samples from the lesions on the face, upper limbs and back of the AD patient group were not clustered by the disease severity. Conclusions The diversity of fungal communities is significantly higher in the lesions on the face, upper limbs and back of the AD patients than in the normal skin at the corresponding body sites of the healthy controls. Malassezia is the dominant fungal genus in both lesions of the AD patients and normal skin of the healthy controls at the above body sites. The composition of fungal communities in lesional samples may be uncorrelated with the disease severity in AD patients.

Key words: Dermatitis, atopic, Skin, Fungi, High-throughput nucleotide sequencing, Biodiversity

引用本文

陆茂 冉玉平 代亚玲 欧美 吴红梅 罗媛元. 特应性皮炎患者面部、上肢和背部皮损真菌群落多样性分析[J]. 中华皮肤科杂志, 2020,53(8):616-622. doi:10.35541/cjd.20191169

Lu Mao, Ran Yuping, Dai Yaling, Ou Mei, Wu Hongmei, Luo Yuanyuan. Diversity of fungal communities on lesions of the face, upper limbs and back in patients with atopic dermatitis[J]. Chinese Journal of Dermatology, 2020, 53(8): 616-622.doi:10.35541/cjd.20191169