中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (2): 83-85.

• 论著 • 上一篇    下一篇

酶联免疫吸附试验检测血清天疱疮抗体的评价

石磊, 张福仁, 周桂芝   

  1. 山东省皮肤病性病防治研究所 济南 250022
  • 收稿日期:2003-02-17 出版日期:2004-02-15 发布日期:2004-02-15

Detection of Serum Anti-desmoglein Autoantibodies in Patients with Pemphigus

SHI Lei, ZHANG Fu-ren, ZHOU Gui-zhi   

  1. Shandong Provincial Institute of Dermatology and Venereology, Jinan 250022, China
  • Received:2003-02-17 Online:2004-02-15 Published:2004-02-15

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摘要： 目的 评价酶联免疫吸附试验(ELISA)用于天疱疮血清学诊断的意义。方法 用ELISA法检测49例天疱疮患者和36例非天疱疮对照者血清,同时以间接免疫荧光法(IIF)检测患者血清抗桥粒芯糖蛋白(Dsg)抗体滴度,比较两者的检测结果。结果 27例寻常型天疱疮(PV)、14例落叶型天疱疮(PF)和6例非天疱疮大疱性疾病患者Dsg1ELISA呈阳性,27例PV、1例PF及1例非天疱疮大疱性疾病患者Dsg3ELISA呈阳性。Dsg1ELISA的敏感性和特异性分别是81.25%和88.89%,Dsg3ELISA的敏感性和特异性分别是81.82%和97.22%。与IIF相比,两者检测结果差异有显著性(χ2=4.31,P<0.05)。结论 ELISA是一种简便、敏感和特异性高的天疱疮血清学诊断方法,并能辅助鉴别PV和PF。

关键词: 酶联免疫吸附测定, 天疱疮, 荧光抗体技术,间接

Abstract: Objective To evaluate the significance of serum anti-desmoglein (anti-Dsg) autoantibodies in the sero-diagnosis of pemphigus. Methods Serum anti-desmoglein autoantibody levels were measured by ELISA and indirect immunofluorescent assay (IIF) in 49 patients with pemphigus, and in 36 non-pemphigus controls. Results Dsg1 antibody was positive by ELISA in 27 of 33 patients with pemphigus vulgaris (PV), 14 of 16 pemphigus foliaceus (PF) and 6 of 36 controls, and Dsg3 antibody was positive by ELISA in 27 of 33 PV, 1 of 16 PF and 1 of 36 controls. The sensitivity and specificity of Dsg1 ELISA were 81.25% and 88.89%, while those of Dsg3 ELISA were 81.82% and 97.22%, respectively. There was a significant difference of positivity rates between ELISA and IIF (χ2=4.31, P<0.05). Conclusions Detection of serum anti-desmoglein autoantibodies by ELISA provides a simple, sensitive and specific assay for the sero-diagnosis of pemphigus, which might be useful for differentiation of PV and PF.

Key words: Enzyme-linked immunosorbent assay, Pemphigus, Fluorescent antibody technique, indirect