中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (12): 881-882.

• 研究报道 • 上一篇    下一篇

玫瑰糠疹患者单一核细胞转录因子T-bet mRNA及GATA-3 mRNA的表达

李玉平1,张晓光2,樊磊强3,王爱学4,马喜兴3,刘爽3   

  1. 1. 河北医科大学第二医院 皮肤性病科
    2. 石家庄河北医科大学第二医院皮肤科
    3. 石家庄市河北医科大学第二医院皮肤科
    4. 石家庄 河北医科大学第二医院皮肤科
  • 收稿日期:2011-01-30 修回日期:2011-03-02 出版日期:2011-12-15 发布日期:2011-12-02
  • 通讯作者: 李玉平 E-mail:lyuping@madmail.com.cn

Expressions of T-bet and GATA-3 mRNA in peripheral blood mononuclear cells of patients with pityriasis rosea

  • Received:2011-01-30 Revised:2011-03-02 Online:2011-12-15 Published:2011-12-02
  • Contact: Yu-Ping LI E-mail:lyuping@madmail.com.cn

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摘要：

目的 探讨T-bet、GATA-3在玫瑰糠疹发病机制中的意义。方法 SYBR Green I实时荧光定量PCR技术检测17例玫瑰糠疹患者和20例正常人外周血单一核细胞中T-bet mRNA和GATA-3 mRNA的表达。结果 玫瑰糠疹患者外周血中T-bet mRNA与 GATA-3 mRNA Avg △Ct值分别为3.33 ± 0.94与5.22 ± 0.69，对照组分别为4.31 ± 1.11与4.36 ± 1.02。患者的T-bet mRNA表达高于对照组（P < 0.05），GATA-3表达低于对照组（P < 0.05）。结论 玫瑰糠疹患者外周血中T-bet/GATA-3升高，Th1优势表达，细胞免疫异常在玫瑰糠疹发病过程中可能具有重要作用。

关键词: 转录因子

Abstract:

Objective To investigate the significance of T-bet and GATA-3 in the pathogenesis of pityriasis rosea. Methods SYBR Green I real-time fluorescence quantitative PCR was performed to detect the expressions of T-bet and GATA-3 mRNA in peripheral blood mononuclear cells （PBMCs） from 17 patients with pityriasis rosea and 20 normal human controls. Results The average delta Ct value of T-bet mRNA and GATA-3 mRNA was 3.33 ± 0.94 and 5.22 ± 0.69 respectively in the patients, 4.31 ± 1.11 and 4.36 ± 1.02 respectively in the controls. There was a higher expression of T-bet mRNA but a lower expression of GATA-3 mRNA in the patients compared with the controls （both P < 0.05）. Conclusions Patients with pityriasis rosea show an increased ratio of T-bet to GATA3 expression and predominant expression of Th1-type cytokines. Abnormal cellular immunity may play an important role in the pathogenesis of pityriasis rosea.