中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (6): 437-440.

• 论著 • 上一篇    下一篇

黄芪甲苷对光老化小鼠皮肤中转化生长因子-β信号通路的影响

李燃1,陈斌2,闫宁3,4,陈刚3,5,李双凤3,4,毕志刚6,张银娣3,7   

  1. 1. 江苏省南京市南京医科大学
    2. 南京医科大学第一附属医院皮肤科
    3.
    4. 南京医科大学
    5. 南京市白下区蓝旗医院
    6. 南京医科大学附属明基医院皮肤科
    7. 南京医科大学临床药理研究室
  • 收稿日期:2010-11-19 修回日期:2011-04-13 出版日期:2011-06-15 发布日期:2011-06-02
  • 通讯作者: 陈斌 E-mail:chenbin3@medmail.com.cn
  • 基金资助:

    国家自然科学基金资助项目;江苏省人事厅“六大人才高峰”资助项目;江苏省人事厅博士后科研基金;中国博士后科学基金面上资助项目

Effects of astragaloside on the transforming growth factor （TGF）-β pathway in photoaged skin of mice

  • Received:2010-11-19 Revised:2011-04-13 Online:2011-06-15 Published:2011-06-02

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摘要：

目的 探讨黄芪甲苷对皮肤光老化的保护机制。方法 BALB/c小鼠分为模型组、模型 + 基质组、模型 + 黄芪甲苷组、正常对照组。RT-PCR测定转化生长因子β受体Ⅱ（TGF-βRⅡ）、Smad 7的mRNA表达水平，免疫组化观察TGF-βRⅡ、Smad 7在小鼠皮肤组织中的蛋白表达情况。结果 正常对照组中，TGF-βRⅡ、Smad 7的灰度值比值分别为0.5688 ± 0.0439、0.5900 ± 0.0585，阳性表达率分别为（53.00 ± 4.72）%、（47.50 ± 3.81）%；模型组中，TGF-βRⅡ、Smad 7的灰度值比值分别为0.2588 ± 0.0283、0.8637 ± 0.0514，阳性表达率分别为（28.20 ± 5.24）%、（82.06 ± 2.18）%；模型 + 基质组中，TGF-βRⅡ、Smad 7的灰度值比值分别为0.2653 ± 0.0456、0.8553 ± 0.0575，阳性表达率分别为（28.74 ± 2.28）%、（82.62 ± 4.02）%；模型 + 黄芪甲苷组中TGF-βRⅡ、Smad 7的灰度值比值分别为0.3767 ± 0.0374、0.7131 ± 0.0410，阳性表达率分别为：（41.64 ± 2.59）%、（64.36 ± 2.62）%。皮肤组织中TGF-βRⅡ和Smad灰度值比值4组小鼠间比较，F值分别为80.98和736.80，TGF-βRⅡ和Smad 7的阳性表达率4组小鼠间比较，F值分别为45.36和132.25，P值均 < 0.01。与正常对照组相比，模型组TGF-βRⅡ mRNA和蛋白表达明显降低，Smad 7 mRNA和蛋白表达显著升高（P值均 < 0.01）。与模型组和模型 + 基质组比较，模型 + 黄芪甲苷组TGF-βRⅡ mRNA和蛋白表达明显升高，Smad 7 mRNA和蛋白表达显著下调（P值均 < 0.01）。模型 + 基质组TGF-βRⅡ、Smad 7的mRNA和蛋白表达与模型组相比差异无统计学意义（P值均 > 0.01）。结论 黄芪甲苷可以通过上调TGF-βRⅡ表达和下调Smad 7表达而改变TGF-β通路的信号转导参与抗光老化。

关键词: 信号转导

Abstract:

Objective To study the protective mechanism of astragaloside on skin photoaging. Methods　BALB/c mice were randomly divided into four groups: model group irradiated with ultraviolet rays （UV）, model plus matrix group pretreated with the matrix before UV irradiation, model plus astragaloside group pretreated with astragaloside 0.08% cream before UV irradiation, normal control group received no irradiation or pretreatment. After 4-week irradiation, the mice were sacrificed, and skin tissues were resected from the back of these mice. Then, reverse transeription PCR （RT-PCR） and immunohistochemistry were performed to detect the mRNA and protein expression of TGF-βRⅡ and Smad 7, respectively. Gray scale ratio was used to represent the mRNA levels of TGF-βRⅡ and Smad 7. Results There was a significant difference in the mRNA level （F = 80.98, 736.80, respectively, both P < 0.01） and protein positivity rate （F = 45.36,132.25, respectively, both P < 0.01） of TGF-βRⅡand Smad 7 among the 4 groups. The mRNA level and protein positivity rate of TGF-βRⅡwere 0.2588±0.0283 and （28.20 ± 5.24）% respectively in the model group, significantly lower than those in the normal control group［0.5688 ± 0.0439, （53.00 ± 4.72）%, both P < 0.01］ and model plus astragaloside group ［0.3767 ± 0.0374, （41.64 ± 2.59）%, both P < 0.01］; on the contrary, the mRNA level and protein positivity rate of Smad 7 in the model group ［0.8637 ± 0.0514, （82.06 ± 2.18）%］ were significantly higher than those in the normal control group ［0.5900 ± 0.0585, （47.50±3.81）%, both P < 0.01］ and model plus astragaloside group ［0.7131 ± 0.0410, （64.36 ± 2.62）%, both P < 0.01］. In the model plus astragaloside group, the mRNA level and protein positivity rate of TGF-βRⅡ were significantly higher than in the model plus matrix group ［0.2653 ± 0.0456, （28.74 ± 2.28）%, both P < 0.01］, while those of Smad 7 were statistically lower than in the model plus matrix group ［0.8553 ± 0.0575, （82.62 ± 4.02）%, both P < 0.01］. However, no significant difference was observed in the mRNA level or protein positivity rate of TGF-βRⅡ or Smad 7 between the model group and model plus matrix group （all P > 0.01）. Conclusion Astragaloside can prevent skin photoaging by the alteration of TGF-β pathway via up-regulating TGF-βRⅡexpression and down-regulating Smad 7 expression.

Key words: Signal transduction