中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (6): 434-436.

• 论著 • 上一篇    下一篇

体内输注CD4+CD25+调节性T细胞对MRL/lps小鼠肾脏损伤的影响

冯林1,刁庆春1,郑峻松2,贺娟3,吴军4   

  1. 1. 重庆市第一人民医院皮肤科
    2.
    3. 第三军医大学药学院临床检验学教研室
    4. 第三军医大学附属新桥医院皮肤科 400037
  • 收稿日期:2010-09-15 修回日期:2010-09-26 出版日期:2011-06-15 发布日期:2011-06-02
  • 通讯作者: 刁庆春 E-mail:qchdiao@vip.sina.com
  • 基金资助:

    重庆市卫生局重点课题

In vivo injection of CD4+CD25+ regulatory T cells affects renal impairment in MRL/lps mice

  • Received:2010-09-15 Revised:2010-09-26 Online:2011-06-15 Published:2011-06-02

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摘要：

目的 探讨CD4+CD25+调节性T细胞（Treg细胞）对MRL/lps小鼠病情进展的影响。方法 实验小鼠分为实验组1、实验组2、对照组共3组，每组8只。采用磁珠分选法分选出MRL/lps小鼠及近交系BALB/c小鼠脾脏中的Treg细胞，浓缩后分别输注入实验组MRL/lps小鼠体内，空白组输注等体积生理氯化钠溶液，3周后测量小鼠尿蛋白、抗-dsDNA抗体的含量，并取肾脏行组织病理及免疫病理检查，比较结果。结果 输注MRL/lps小鼠Treg细胞的实验组1，尿蛋白评分显著低于输注正常小鼠Treg细胞组（实验组2）及对照组，分别为10.63 ± 4.17、20.00 ± 5.35、18.75 ± 8.34；P < 0.05。抗-dsDNA抗体的含量显著低于实验组2及对照组，分别为（5.36 ± 2.40） pg/ml、（9.57 ± 1.97） pg/ml、（10.75 ± 3.98） pg/ml；P < 0.05。肾小球硬化指数3组分别为（32.00 ± 12.09）%、（45.50 ± 13.68）%、（47.50 ± 10.78）%；P < 0.05。肾脏中IgG复合物的免疫荧光评分，3组分别为1.88 ± 0.99、2.88 ± 0.64、2.75 ± 0.71；P < 0.05，实验1组均显著轻于另外两组。肾小管间质损害指数三组间差异无统计学意义，3组分别为4.63 ± 1.92、6.00 ± 1.07、5.75 ± 1.28；P > 0.05）。结论 输注同系小鼠的Treg细胞可显著降低尿蛋白及抗dsDNA抗体的含量，降低肾小球硬化指数及肾脏中IgG免疫复合物的含量，从而延缓MRL/lps小鼠肾脏损伤的进展。

关键词: 肾损伤

Abstract:

Objective To investigate the influence of CD4+CD25+ regulatory T cells（Tregs） on the disease progression in MRL/lps mice. Methods Tregs were separated by using magnetic beads from splenic cells of MRL/lps mice and BALB/c mice, and concentrated. Twenty-four MRL/lps mice were equally divided into 3 groups, test group 1 injected with Tregs from MRL/lps mice, test group 2 injected with Tregs from BALB/c mice, and control group injected with physiological sodium chloride solution. Three weeks later, the levels of urine protein as well as serum anti-dsDNA antibody were determined; subsequently, the mice were sacrificed followed by histopathological and immunopathological examination of renal tissue. Results A significant decline was observed in the test group 1 compared with the test group 2 and control group in the urine protein score （10.63 ± 4.17 vs. 20.00 ± 5.35 and 18.75 ± 8.34, both P < 0.05）, serum anti-dsDNA antibody level （5.36 ± 2.40 pg/ml vs. 9.57 ± 1.97 pg/ml and 10.75 ± 3.98 pg/ml, both P < 0.05）, glomerular sclerosis index ［（32.00 ± 12.09）% vs. （45.50 ± 13.68）% and （47.50 ± 10.78）%, both P < 0.05］, and immunofluorescence intensity of IgG immune complex in renal tissue （1.88 ± 0.99 vs. 2.88 ± 0.64 and 2.75 ± 0.71, both P < 0.05）. No significant difference was noted in renal tubule interstitial impairment index between the 3 groups（4.63 ± 1.92, 6.00 ± 1.07 and 5.75 ± 1.28, all P > 0.05）. There was no statistical difference between the test group 2 and control group in terms of any of the above parameters （all P > 0.05）. Conclusions Injection of Tregs from homologous mice could significantly down-regulate proteinuria degree, serum anti-dsDNA antibody level, glomerular sclerosis index and IgG immune complex level in renal tissue, and thereby decelerate the progression of renal impairment in MRL/lps mice.

Key words: renal impairment