中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (6): 441-442.

• 研究报道 • 上一篇    下一篇

儿童头皮与包皮组织诱导前后犬小孢子菌FSH1的表达

祝逸平1,张振颖2,庞娟1,杨国玲3   

  1. 1.
    2. 大连医科大学附属第一医院皮肤科教研室
    3. 大连医科大学附属第一医院
  • 收稿日期:2010-08-19 修回日期:2010-12-16 出版日期:2011-06-15 发布日期:2011-06-02
  • 通讯作者: 杨国玲 E-mail:yangg@medmail.com.cn
  • 基金资助:

    利用抑制性消减杂交技术探讨犬小孢子菌蛋白酶在脓癣发病中的作用

Expression of FSH1 in Microsporum canis before and after the induction by skin tissue from the scalp and foreskin of children

  • Received:2010-08-19 Revised:2010-12-16 Online:2011-06-15 Published:2011-06-02

PDF

17

赞

摘要：

目的 探讨丝氨酸水解酶（FSH1）在犬小孢子菌致病中的意义。方法 半定量RT-PCR检测30株犬小孢子菌头癣株和30株犬小孢子菌体癣株经沙氏液体培养基传代及儿童头皮、包皮诱导前后FSH1的表达。结果 头癣株犬小孢子菌FSH1mRNA表达水平明显高于体癣株（P < 0.01），经沙氏液体培养基传至第5代的菌株FSH1mRNA表达水平较第一代差异无统计学意义（P > 0.05）。经儿童头皮或包皮诱导后，FSH1mRNA表达水平较诱导前明显升高（F = 2025.713、1833.139，P 值均 < 0.01），且不同皮肤组织（儿童头皮、儿童包皮）诱导后的菌株其表达水平差异有统计学意义（P < 0.01）。结论 不同感染部位的犬小孢子菌FSH1mRNA表达水平不同；局部组织对该菌的FSH1mRNA表达存在诱导作用，且儿童头皮的诱导作用明显。

关键词: 半定量RT-PCR

Abstract:

Objective To explore the significance of FSH1 in the pathogenicity of M. canis. Methods Thirty M. canis strains from tinea capitis lesions and 30 M. canis strains from tinea corporis lesions were cultured, passaged, and induced by medium containing skin tissue of scalp or foreskin from children. Semi-quantitative reverse transcription （RT）-PCR was carried out to detect the expression of FSH1 mRNA in the first- and fifth-generation M. canis strains, as well as M. canis strains induced by the skin tissues. Results The mRNA expression of FSH1 was higher in M. canis strains derived from tinea capitis lesions than in those from tinea corporis lesions（P < 0.01）, but there was no significant difference between the first-generation and fifth-generation M. canis strains（P > 0.05）. The skin tissue from scalp and foreskin induced a significant elevation in the mRNA expression of FSH1 in these M. canis strains （F = 2025.713, 1833.139, both P < 0.01）, and the inductive effect of the scalp tissue was different from that of the foreskin tissue （P < 0.01）. Conclusions The FSH1 mRNA expression is different in M. canis isolated from different body sites. Local skin tissue has an inductive effect on the expression of FSH1 mRNA, and the inductive effect of scalp tissue is more apparent than that of foreskin tissue.

Key words: semi-quantitative RT-PCR