中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (10): 702-704.

• 论著 • 上一篇    下一篇

TLR2和TLR9在生殖器疱疹患者外周血单一核细胞的表达

汤建萍1,杨于嘉2,孙磊3,等2   

  1. 1. 长沙市湖南省儿童医院皮肤性病科
    2.
    3. 长沙 湖南省儿童医院皮肤科
  • 收稿日期:2008-10-04 修回日期:2008-12-11 发布日期:2009-10-09
  • 通讯作者: 汤建萍 E-mail:jpingtang@yahoo.com.cn,jpingtang@hotmail.com
  • 基金资助:

    市(厅)级基金（编号）

Expressions of Toll-like receptor （TLR） 2 and TLR9 in peripheral blood mononuclear cells from patients with herpes zoster

  • Received:2008-10-04 Revised:2008-12-11 Published:2009-10-09

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摘要：

目的 探讨Toll样受体TLR2和TLR9在生殖器疱疹中的作用。方法 流式细胞仪检测生殖器疱疹患者单一核细胞上TLR2和TLR9表达水平，同时采用ELISA法检测其血清IL-6、IL-10和TNF-α水平，并探讨其相关性。结果 ①生殖器疱疹患者PBMC上TLR2和TLR9表达水平明显高于正常成人（P ＜ 0.05）。②生殖器疱疹患者血清IL-6 和IL-10水平明显升高，TNF-α水平明显降低，与对照组比较，差异有统计学意义（P ＜ 0.05）。③生殖器疱疹患者TLR2和TLR9表达水平与其血清IL-6，IL-10水平存在线性相关关系（P ＜ 0.01）。结论 TLR2和TLR9介导生殖器疱疹血清IL-6、IL-10的产生和释放。

关键词: 单纯疱疹, 外周血单个核细胞, Toll 样受体, 细胞因子

Abstract:

Objective To study the expression level of TLR2 and TLR9 in peripheral blood mononuclear cells （PBMC） from patients with genital herpes （GH）. Methods Venous blood samples were collected from 20 patients with GH and 10 normal human controls. Flow cytometry was used to detect the expression levels of TLR2 and TLR9 in PBMC from these samples. Serum levels of IL-6, IL-10 and TNF-α were measured by ELISA. Results The percentage of PBMCs expressing TLR2 or TLR9 was significantly higher in patients than in normal human controls （18.88% ± 15.31% vs 7.49% ± 1.55%, 85.29% ± 3.15% vs 22.23% ± 2.38%, both P < 0.05）. Compared with the normal human controls, increased serum levels of IL-6 and IL-10 were observed in patients （91.25 ± 3.22 pg/ml vs 6.65 ± 1.27 pg/mL, 3.94 ± 0.57 pg/mL vs 2.13 ± 0.24 pg/mL, both P < 0.05）, together with a decrease in the serum level of TNF-α （12.07 ± 1.27 pg/mL vs 23.06 ± 4.62 pg/mL）. The expression levels of TLR2 and TLR9 significantly correlated with serum levels of IL-6 and IL-10 in patients with GH （all P < 0.01）. Conclusion The production and release of serum IL-6 and IL-10 might be mediated by TLR2 and TLR9 in patients with genital herpes.