中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (11): 790-792.

• 论著 • 上一篇    下一篇

抗胆碱酯酶药对寻常型天疱疮鼠模型棘层松解的影响

韦高1,郑文军2,周琼3   

  1. 1. 南宁市广西医科大学一附院皮肤科
    2. 南宁广西医科大学第一附属医院皮肤科
    3. 江西省南昌市第一医院
  • 收稿日期:2013-10-28 修回日期:2014-05-14 出版日期:2014-11-15 发布日期:2014-11-01
  • 通讯作者: 郑文军 E-mail:360887519@qq.com

Effect of anticholinesterase agents on acantholysis in a mouse model of pemphigus vulgaris

1, 1,qiong ZHOU2   

  1. 1.
    2. The First Hospital of Nanchang
  • Received:2013-10-28 Revised:2014-05-14 Online:2014-11-15 Published:2014-11-01

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摘要： 目的 通过鼠模型研究抗胆碱酯酶药对寻常型天疱疮（PV）棘层松解的影响。 方法 将55只Balb/c新生鼠分为4组，模型组：15只，将PV患者血清通过皮下注射BALB/c新生鼠建立PV鼠模型；溴吡斯的明组、甲硫酸新斯的明组：各15只，在注射患者血清的同时，分别注射溴吡斯的明及甲硫酸新斯的明；对照组：10只，皮下注射生理氯化钠溶液。从临床表现、组织病理和直接免疫荧光三方面评价抗胆碱酯酶药对PV鼠棘层松解的影响。 结果 模型组单独注射PV患者血清能在BALB/c新生鼠中诱导出PV的改变。模型组棘层松解程度高于溴吡斯的明组（H = 21.584，P ＜ 0.001）及甲硫酸新斯的明组（H = 20.641，P ＜ 0.001），对照组BALB/c新生鼠未发生明显变化。 结论 抗胆碱酯酶药可降低PV鼠模型的棘层松解程度。

关键词: 天疱疮, 疾病模型，动物, 胆碱酯酶抑制剂

Abstract: Wei Gao, Zheng Wenjun, Zhou Qiong. Department of Dermatology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China Corresponding author: Zheng Wenjun, Email: 591292777@qq.com 【Abstract】 Objective To study the effect of anticholinesterase agents on acantholysis in pemphigus vulgaris （PV） by using a mouse model. Methods Fifty-five neonatal BALB/c mice were divided into four groups: model group injected subcutaneously with the sera of patients with PV （n = 15）, pyridostigmine bromide group （n = 15） and neostigmine methylsulfate group （n = 15） subcutaneously injected with pyridostigmine bromide and neostigmine methylsulfate respectively, in addition to the sera of PV patients, control group subcutaneously injected with sodium chloride physiological solution （n = 10）. The effect of anticholinesterase agents on acantholysis in PV was evaluated in terms of clinical presentation, histopathological manifestations and direct immunofluorescence findings. Results The injection of sera from PV patients induced characteristic changes of PV in neonatal BALB/c mice in the model group. The degree of acantholysis in the model group was higher than that in the pyridostigmine bromide group （H = 21.584, P < 0.001） and neostigmine methylsulfate group （H = 20.641, P < 0.001）. No changes were observed in the control group. Conclusion Anticholinesterase agents can reduce the degree of acantholysis in the mouse model of PV.

Key words: Pemphigus, Disease models, animal, Cholinesterase inhibitors