中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (11): 808-809.

• 研究报道 • 上一篇    下一篇

斑秃患者外周血Th17细胞的检测

柳小婧1,徐峰2,韩毓梅3,4,盛友渔2,缪盈3,4,胡瑞铭4,郭霞5,齐思思5,杨勤萍6   

  1. 1. 上海市皮肤病医院
    2. 上海复旦大学附属华山医院皮肤科
    3.
    4. 复旦大学附属华山医院
    5. 复旦大学附属华山医院皮肤科
    6. 复旦大学附属华山医院皮肤科,复旦大学附属华山医院南汇分院
  • 收稿日期:2014-01-01 修回日期:2014-02-11 出版日期:2014-11-15 发布日期:2014-11-01
  • 通讯作者: 杨勤萍 E-mail:dxq93216@medmail.com.cn
  • 基金资助:
    中华医学会皮肤性病学分会美能皮肤病学研究基金

Detection of T helper type 17 cells in peripheral blood of patients with alopecia areata

  • Received:2014-01-01 Revised:2014-02-11 Online:2014-11-15 Published:2014-11-01
  • Contact: YANG Qinping E-mail:dxq93216@medmail.com.cn

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摘要： 目的 分析Th17细胞与斑秃病程、病情等的关系。 方法 分别抽取斑秃组和健康对照组的外周血，流式细胞仪检测Th17细胞的比例，用SPSS16.0进行统计分析。 结果 斑秃组60例外周血中Th17细胞占CD4+ T细胞的比例（1.33% ± 0.74%）显著高于30例健康对照组（0.91% ± 0.54%，P ＜ 0.01）。初发斑秃患者Th17细胞比例显著高于复发患者（P ＜ 0.05），活动期高于非活动期（P ＜ 0.05），病程 ≤ 6个月者高于病程 ＞ 6个月者（P ＜ 0.01），而头皮脱发面积 ≤ 50%者与脱发面积 ＞ 50%及全秃和普秃者比较差异无统计学意义。 结论外周血Th17细胞的水平与斑秃活动性、疾病复发及病程有关。

关键词: 斑秃, 自身免疫, Th17细胞

Abstract: Liu Xiaojing, Xu Feng, Han Yumei, Sheng Youyu, Miao Ying, Hu Ruiming, Guo Xia, Qi Sisi, Yang Qinping. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China Corresponding author: Yang Qinping, Email: dxq93216@medmail.com.cn 【Abstract】 Objective To investigate the relationship of peripheral blood T helper type 17 （Th17） cells with the clinical course and severity of alopecia areata （AA）. Methods Peripheral blood samples were obtained from 60 patients with AA and 30 healthy human controls. Flow cytometry was performed to determine the proportion of Th17 subsets in these samples. Statistical analysis was done by two independent samples t-test with SPSS software version 16.0. Results The proportion of Th17 subsets in peripheral blood CD4+ T cells was significantly higher in the AA patients than in the healthy controls （1.33% ± 0.74% vs. 0.91% ± 0.54%, P ＜ 0.01）, higher in patients with initial AA than in those with recurrent AA （P ＜ 0.05）, higher in patients with active AA than in those with inactive AA （P ＜ 0.05）, higher in patients with a clinical course of < or = 6 months than in those > 6 months （P ＜ 0.01）. No significant difference was observed in the proportion of peripheral blood Th17 cells between patients with AA involving no more than 50% of the scalp and those with AA involving more than 50% of the scalp, alopecia totalis or alopecia universalis （P > 0.05）. Conclusion The level of peripheral blood Th17 cells is associated with the activity, relapse, and clinical course of AA.

Key words: Alopecia areata, Autoimmunity, Th17 cells