中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (6): 421-423.

• 研究报道 • 上一篇    下一篇

Pinx1、hTERT mRNA在三种皮肤肿瘤组织中的表达及意义

陈前明1,方杰2,瓦庆彪3,袁伟4,吴波3,蔡琦1,路永红1,周培媚1,蒋存火1   

  1. 1. 成都市第二人民医院皮肤科
    2. 遵义医学院附属医院
    3. 成都市第二人民医院
    4. 遵义医学院附属医院皮肤科
  • 收稿日期:2013-08-19 修回日期:2013-09-25 发布日期:2014-06-01
  • 通讯作者: 袁伟 E-mail:dryuanweihua@sina.com
  • 基金资助:
    四川省科技支撑计划项目

Expressions of PinX1 and hTERT mRNAs in three skin tumors and their significance

  • Received:2013-08-19 Revised:2013-09-25 Published:2014-06-01

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摘要： 目的 探讨内源性端粒酶抑制基因PinX1及端粒酶逆转录酶hTERT在基底细胞癌、鳞状细胞癌、日光性角化病组织中的表达及其意义。 方法 实时定量PCR检测实验组36例3种皮肤肿瘤组织中内源性端粒酶抑制基因PinX1及端粒酶逆转录酶hTERT的mRNA表达水平，并以15例正常人皮肤组织作为对照组。 结果 实验组PinX1基因的mRNA表达水平（2.53 ± 1.57）低于对照组（4.25 ± 2.02），差异有统计学意义（t = 3.273，P < 0.05）；hTERT基因的mRNA表达水平（6.37 ± 3.81）明显高于对照组（1.62 ± 0.99），差异有统计学意义（t = 6.927，P < 0.05）；Pinx1的表达水平与hTERT表达的相关性无统计学意义（P > 0.05）。 结论 PinX1的下调及hTERT上调可能与3种皮肤肿瘤形成过程中端粒酶激活及维持机制有关。

关键词: 皮肤肿瘤, 基因，Pinx1, 基因，hTERT

Abstract: Chen Qianming*, Fang Jie, Wa Qingbiao, Yuan Wei, Wu Bo, Cai Qi, Lu Yonghong, Zhou Peimei, Jiang Cunhuo. *Department of Dermatology, Second People′s Hospital of Chengdu, Chengdu 610017, China Corresponding author: Yuan Wei, Email: dryuanweihua@sina.com 【Abstract】 Objective To investigate the mRNA expressions of PinX1 and hTERT in lesions of three skin tumors （including basal cell carcinoma （BCC）, squamous cell carcinoma （SCC） and actinic keratosis） as well as their significance. Methods This study included 36 tissue specimens from the lesions of 13 patients with BCC, 11 patients with SCC and 12 patients with actinic keratosis, as well as 15 tissue specimens from the normal skin of 15 individuals receiving surgical therapy. Real-time PCR was performed to quantify the mRNA expressions of PinX1 and hTERT in these specimens. Results The specimens from the lesions of the skin tumors showed a significantly lower expression of PinX1 mRNA （2.53 ± 1.57 vs. 4.25 ± 2.02, t = 3.273, P < 0.05）, but a higher expression of hTERT mRNA （6.37 ± 3.81 vs. 1.62 ± 0.99, t = 6.927, P < 0.05） compared with those from the normal skin. There was no significant correlation between the mRNA expressions of PinX1 and hTERT （P > 0.05）. Conclusion The down-regulated PinX1 expression and up-regulated hTERT expression may be associated with the activation and maintenance of telomerase in the formation of the three skin tumors.

Key words: Skin neoplasms, Genes, Pinx1, Genes, hTERT

引用本文

陈前明 方杰 瓦庆彪 袁伟 吴波 蔡琦 路永红 周培媚 蒋存火. Pinx1、hTERT mRNA在三种皮肤肿瘤组织中的表达及意义[J]. 中华皮肤科杂志, 2014,47(6):421-423. doi: