中华皮肤科杂志

• 论著 • 上一篇    下一篇

维胺酯对HaCaT细胞增殖和分化的影响

陈翔, 罗婷婷, 粟娟, 张蓓蓓   

  1. 中南大学湘雅医院皮肤科, 长沙410008
  • 收稿日期:2006-12-13 出版日期:2007-11-15 发布日期:2007-11-15

Effects of viaminate on the proliferation and differentiation of a human keratinocyte cell line HaCaT

CHEN Xiang, LUO Ting-ting, SU Juan, ZHANG Bei-bei   

  1. Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China
  • Received:2006-12-13 Online:2007-11-15 Published:2007-11-15

PDF (PC)

92

赞

摘要： 目的 探讨维胺酯对体外培养角质形成细胞(HaCaT细胞)增殖和分化的影响.方法 将浓度为2,5,10,15,20,25,30 μg/mL的维胺酯作用于培养的HaCaT细胞,采用MTT法检测维胺酯对HaCaT细胞体外增殖的影响,流式细胞仪测定细胞周期及凋亡率变化,逆转录-聚合酶链反应(RT-PCR)半定量检测分化标记物角蛋白10及内披蛋白mRNA的表达水平.结果 2 μg/mL维胺酯处理的HaCaT细胞,48 h时表现出对细胞增殖的抑制作用,随着时间延长和药物剂量加大,抗增殖作用愈明显;当药物质量浓度达到30 μg/mL时,48 h和72 h时的抑制率分别为57.67%和82.00%.与对照组相比,经维胺酯作用48 h后,细胞G1期比例显著增加,S期与G2期比例则显著下降,并可抑制G1/G2期转换,但对细胞凋亡无影响.细胞内披蛋白mRNA表达水平随维胺酯处理浓度增高而上升,药物浓度达30μg/mL时,表达水平由对照组的40.80%增高至156.12%;而角蛋白10 mRNA表达水平则下降,由96.46%降至14.60%.结论 维胺酯具有抑制角质形成细胞增殖及诱导其分化的作用.

关键词: 维生素A酸类, 角蛋白细胞, 细胞增殖, 细胞分化

Abstract: Objective To investigate the effects ofviaminate on the proliferation and differentiation of HaCaT cells,a human keratinocyte cell line.Methods Cultured HaCaT cells were treated with various concentrations (2,5,10,15,20,25 and 30μg/mL) of viaminate for various durations.The cell proliferation was assessed by MTT method,the changes of cell cycle and apoptosis rate by flow cytometry,the changes of keratin 10 and involucrin mRNA expressions by semi-quantitative reverse transcription PCR.Results The proliferation of HaCaT cells was inhibited by the treatment with viaminate of≥2μg/mL for 48 h,and the inhibition rate was raised with the increase of treatment time and dosage.The viaminate of 30μg/mL inhibited the proliferation of HaCaT cells by 57.67% and 82.00% at 48 and 72 h after the incubation respectively,and elevated the mRNA expression of involucrin from 40.80% to 156.12%,decreased the mRNA expression of keratin 10 from 96.46% to 14.60%.The mRNA expression of involucrin increased with the elevation of viarninate dosage.Under the treatment with viaminate for 48 h,the cell population at G1 phase significantly increased,that at S and G2 phases decreased;the switching of G1 to G2 was inhibited;but the cell apoptosis was not affected.Conclusion Viaminate could inhibit the proliferation and induce the differentiation of keratinocytes.

Key words: Retinoids, Keratinocytes, Cell proliferation, Cell differentiation