中华皮肤科杂志

• 论著 • 上一篇    下一篇

蛋白质芯片对莱姆病患者血清抗鞭毛蛋白抗体的检测分析

杜卫东1, 何素敏2, 张学军2   

  1. 1. 安徽医科大学皮肤病研究所, 合肥230032;
    2. 安徽医科大学重要遗传病基因资源重点实验室
  • 收稿日期:2006-12-13 出版日期:2007-11-15 发布日期:2007-11-15
  • 作者简介:杜卫东,email:weidongdu@hotmail.com

Detection of serum antibodies against flagellin with a protein biochip in patients with Lyme borre-liosis

DU Wei-dong1, HE Su-min2, ZHANG Xue-jun2   

  1. Department of Dermatology, First Affiliated Hospital of Anhui Medical University, Hefei 230032, China
  • Received:2006-12-13 Online:2007-11-15 Published:2007-11-15

PDF (PC)

167

赞

摘要： 目的 探讨蛋白质芯片在莱姆病诊断中的价值.方法 用伯氏疏螺旋体鞭毛蛋白包被N-羟基琥珀酰亚胺修饰的芯片对82例神经性莱姆病、35例环形红斑莱姆病患者和44例正常人对照血清鞭毛蛋白特异性IgM和IgG抗体进行检测,并与酶联免疫吸附(ELISA)和蛋白质印迹方法比较.结果 神经性莱姆病患者血清IgM和IgG抗体水平均高于正常人对照组(P<0.001).蛋白质芯片检测神经性莱姆病患者IgM抗体阳性率为76.8%,ELISA检测为63.4%,两种方法检测的阳性率比较,差异有统计学意义(P<0.05).蛋白质芯片、ELISA和蛋白质印迹联合检测使环形红斑莱姆病患者IgM和IgG抗体阳性率有所提高.对蛋白质芯片和ELISA两种方法检测的神经性莱姆病、环形红斑莱姆病患者IgM抗体结果分别进行线性分析,发现两种检测方法具有相关性.结论 鞭毛蛋白包被N-羟基琥珀酰亚胺修饰的蛋白质芯片可用于莱姆病临床诊断.

关键词: 莱姆病, 蛋白质阵列分析, 鞭毛蛋白, 伯氏疏螺旋体

Abstract: Objective To investigate the value of protein biochip in the diagnosis of Lyme borreliosis. Methods The serum IgM and IgG antibodies against flagellin were detected by flagellin coated-and N-hydroxysuccinimide (NHS) modified-biochips in 82 patients with neuroborreliosis (NB),35 patients with erythema migrans (EM) and 44 normal controls.The results were compared with those from enzyme-linked immunosorbent assays (ELISA) and Western blot.Results The levels of both IgM and IgG antibodies were significantly higher in the NB patients than those in the normal controls (P<0.001).The frequency of IgM antibody detection was significantly higher with biochip than that with ELISA (76.8% vs 63.4%, P<0.05).With the application of the combination of protein biochip,ELISA and Western blot,the detection rates of IgM and IgG antibodies against flagellin were increased in EM patients.The detection results of IgM antibodies were significantly correlated between protein biochip and ELISA in EM and NB patients. Conclusion The flagellin coated-and NHS modified-biochip could be potentially applied in clinical immunodiagnosis of Lyme borreliosis.

Key words: Lyme disease, Protein array analysis, Flagellin, Borrelia burgdorferi