中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (7): 462-465.

• 论著 • 上一篇    下一篇

特应性皮炎患者外周血调节性T细胞及皮损中miR-31、Foxp3的表达

马蕾1,薛海波2,管秀好3,运蓓蕾4,5,王娟2,5,张俊花6,安荣真6,张玉杰6   

  1. 1. 滨州医学院附属医院皮肤科
    2. 滨州医学院附属医院
    3. 沈阳 中国医科大学附属第一医院皮肤科
    4. 沈阳市第七人民医院
    5.
    6. 山东省滨州医学院附院皮肤科
  • 收稿日期:2012-08-13 修回日期:2012-08-21 出版日期:2013-07-15 发布日期:2013-07-01
  • 通讯作者: 薛海波 E-mail:xuehaibo@sina.com
  • 基金资助:
    山东省2010年科学技术发展计划;山东省自然科学基金;山东省高等学校科技计划项目;山东省医药卫生科技发展计划项目;滨州市科技发展计划

Proportion of peripheral blood regulatory T cells as well as mRNA expressions of miR-31 and Foxp3 in peripheral blood and skin lesions from patients with atopic dermatitis

  • Received:2012-08-13 Revised:2012-08-21 Online:2013-07-15 Published:2013-07-01
  • Supported by:
    ;Projects of Medical and Health Technology Development Program in Shandong Province

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摘要： 目的 探讨特应性皮炎（AD） 患者外周血调节性T细胞（Treg 细胞）及皮损组织中miR-31、Foxp3的表达水平及其相关性。方法 用流式细胞仪检测37例AD患者外周血Treg细胞的比例，实时荧光定量RT-PCR检测miR-31及Treg细胞特异性转录因子Foxp3 mRNA的表达水平；并以33例性别、年龄匹配的健康儿童做对照。检测5例急性期AD患者皮损与皮损周围组织中miR-31及Foxp3 mRNA的表达水平，并以5例健康人皮肤组织标本为对照。采用独立样本t检验、单因素方差分析和线性相关进行统计学分析。结果 AD患者外周血Treg细胞比例明显低于健康对照组（2.12% ± 0.60%比4.99% ± 1.27%，P < 0.01）；miR-31 mRNA表达水平明显高于健康对照组（6.01 ± 1.76比1.62 ± 0.51，P < 0.01）；Foxp3 mRNA表达水平显著低于健康对照组（0.78 ± 0.17比1.87 ± 0.71，P < 0.01）。AD患者皮损、皮损周围组织及健康人皮肤组织中miR-31及Foxp3 mRNA表达水平间差异均有统计学意义。miR-31表达水平：皮损 > 皮损周围组织 > 健康人皮肤组织，F = 54.501，P < 0.01；Foxp3表达水平：皮损 < 皮损周围组织 < 健康人皮肤组织，F = 37.837，P < 0.01。AD患者外周血miR-31 mRNA表达水平与疾病严重程度评分呈正相关（r = 0.417，P = 0.010），与Treg细胞比例（r = -0.404，P = 0.013）及Foxp3 mRNA表达水平呈负相关（r = -0.409，P = 0.012）；皮损及皮损周围组织中miR-31 mRNA表达水平与Foxp3 mRNA表达水平呈负相关（r = -0.392，P = 0.032）。结论 AD患者miR-31高表达及Foxp3低表达可能与AD的发病相关。 【关键词】 皮炎，特应性； miR-31； T淋巴细胞，调节性； Foxp3

关键词: 皮炎，特应性, miR-31, T淋巴细胞，调节性, Foxp3

Abstract: MA Lei *, XUE Hai-bo, GUAN Xiu-hao, YUN Bei-lei, WANG Juan, ZHANG Jun-hua, AN Rong-zhen, ZHANG Yu-jie. *Department of Dermatology, Affiliated Hospital of Binzhou Medical University, Binzhou 256603, Shandong, China Corresponding author: XUE Hai-bo, Email: xuehaibo@sina.com 【Abstract】 Objective To determine the proportion of peripheral blood regulatory T （Treg） cells as well as mRNA expressions of miR-31 and Foxp3 in peripheral blood and skin lesions from patients with atopic dermatitis （AD）, and to assess their association. Methods Peripheral blood samples were obtained from 37 patients with AD and 33 age- and sex-matched healthy controls, and biopsy samples from the lesional and perilesional skin of 5 patients with AD as well as from the normal skin of 5 healthy controls. Flow cytometry was performed to determine the percentage of Treg cells （CD4+CD25+Foxp3+ T cells） in peripheral blood, and fluorescence-based real time quantitative reverse transcription （RT）-PCR to quantify the mRNA expressions of miR-31 and Foxp3 （a Treg cell-specific transcription factor） in peripheral blood and skin samples. Independent samples t test was carried out to compare the percentage of Treg cells as well as the mRNA expressions of miR-31 and Foxp3 in peripheral blood between the patients and controls, one-way analysis of variance to compare the mRNA expressions of miR-31 and Foxp3 in biopsy samples between the patients and controls, and Pearson correlation analysis to evaluate the relationship between miR-31 mRNA expression level, SCORing Atopic Dermatitis （SCORAD） score, Treg cell percentage, and Foxp3 mRNA expression level. Results The patients with AD showed decreased Treg cell percentage （2.12% ± 0.60% vs. 4.99% ± 1.27%，P < 0.01） and Foxp3 mRNA expression level（0.78 ± 0.17 vs. 1.87 ± 0.71, P < 0.01）, but increased miR-31 mRNA expression level（6.01 ± 1.76 vs. 1.62 ± 0.51, P < 0.01）in peripheral blood compared with the healthy controls. The expression level of miR-31 mRNA sequentially decreased（F = 54.501, P < 0.01）, but the expression of Foxp3 mRNA sequentially increased （F = 37.837, P < 0.01） from lesional skin, perilesional skin to normal skin tissues. The miR-31 mRNA expression level was positively correlated with SCORAD score （r = 0.417, P = 0.010）, but negatively correlated with Treg cell percentage （r = －0.404, P = 0.013） and Foxp3 mRNA level （r = －0.409, P = 0.012） in peripheral blood of patients with AD. There was a negative correlation between the mRNA expression level of miR-31 and Foxp3 in lesional and peri-lesional skin tissues of the patients （r = －0.392, P = 0.032）. Conclusion The upregulated miR-31 expression and downregulated Foxp3 expression may be associated with the development of AD. 【Key words】 Dermatitis, atopic; miR-31; T-lymphocytes, regulatory; Foxp3

Key words: miR-31, Foxp3