中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (9): 527-529.

• 论著 • 上一篇    下一篇

香烟烟雾提取物对培养的人皮肤成纤维细胞生长和增殖的影响

钱革1, 周武庆1, 武欣生2, 王千秋1, 万毅3, 钟铭英1, 蒋法兴1, 张国成1   

  1. 1. 中国医学科学院皮肤病研究所, 南京 210042;
    2. 南京医科大学附属儿童医院;
    3. 第四军医大学统计学教研室
  • 收稿日期:2006-10-18 出版日期:2007-09-15 发布日期:2007-09-15
  • 通讯作者: 王千秋, email:wangqianqiu@yahoo.com.cn E-mail:wangqianqiu@yahoo.com.cn
  • 基金资助:
    2007年中华医学会一欧莱雅健康皮肤/健康毛发研究项目资助(S2007040809)

Effects of cigarette smoke extract on cell proliferation and growth of cultured human skin fibroblasts

QIAN Ge1, ZHOU Wu-qing1, WU Xin-sheng2, WANG Qian-qiu1, WAN Yi3, ZHONG Ming-ying1, JIANG Fa-xing1, ZHANG Guo-cheng1   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Nanjing 210042, China
  • Received:2006-10-18 Online:2007-09-15 Published:2007-09-15

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摘要： 目的 探讨香烟烟雾提取物(CSE)对原代培养的皮肤成纤维细胞生长和增殖的影响.方法 采用噻唑蓝(MTT)比色法检测体外皮肤成纤维细胞存活力的影响,观察染毒后细胞形态和结构变化. β-半乳糖苷酶染色方法检测染毒后多次传代后成纤维细胞 β-半乳糖苷酶的变化情况.用分光光度法检测胞质内活性氧自由基(ROS)含量、超氧化物歧化酶(SOD)活性.结果 染毒后细胞呈典型的损伤形态,随着CSE浓度的增大和作用时间的延长,细胞存活力下降.长期染毒的细胞经5次传代后, β-半乳糖苷酶呈显著阳性;生长曲线示CSE作用后的细胞生长速度明显减慢.CSE作用于细胞后,细胞质内ROS含量升高(P<0.05),SOD活性降低(P<0.05).结论 CSE对体外培养的皮肤成纤维细胞增殖和生长有一定的抑制作用.低浓度CSE长期作用,可以促使原代培养的成纤维细胞迅速衰老.

关键词: 烟, 烟雾提取物, 成纤维细胞, 生长

Abstract: Objective To study the effect of cigarette smoke extract(CSE) on the proliferation and growth of primarily cultured skin fibroblasts.Methods Fibroblasts were isolated from the foreskin of healthy young adults,and subjected to primary culture.After 5～15 passages,these fibroblasts were exposed to various concentrations of CSE for various durations.MTT assay was used to detect the viability of fibroblasts after exposure.The morphological and structural changes of these cells were observed by microscopy. β-galactosidase staining was performed on fibroblasts treated with CSE during several passages.Growth curves were drawn with MTT method for fibroblasts exposed to low concentration of CSE during 5 passages.The content of reactive oxygen species(ROS) in cell plasma,and activities of superoxide dismutase(SOD) in cells were determined by spectrophotometric method.Results The fibroblasts displayed typical morphological changes after exposed to CSE for 24 h.The viability of fibroblasts declined with the increase of CSE concentration and treatment time. β-galactosidase was detected more frequently in fibroblasts exposed to CSE during 5 passages than in those without any treatment.After CSE treatment,the content of ROS significantly increased,while the activities of SOD decreased(both P<0.05).Conclusions These findings suggest that CSE could injure cultured fibroblasts and inhibit the growth and proliferation of them.Furthermore, long-term action of CSE at low concentration could accelerate the senescence of primarily cultured fibroblasts,and oxidative damage may be involved in the CSE injury to cells.

Key words: Smoke, Smog extract, Fibroblasts, Growth