中华皮肤科杂志

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白念珠菌临床株多药耐药蛋白基因表达与氟康唑耐药的关系

秦晓峰1, 吴建华2, 黄懿1, 姜远英3, 顾军2   

  1. 1. 同济大学附属东方医院皮肤科 上海 200120;
    2. 第二军医大学附属长海医院皮肤科 上海 200433;
    3. 第二军医大学药学院药理学教研室
  • 收稿日期:2006-12-08 出版日期:2007-08-15 发布日期:2007-08-15
  • 通讯作者: 顾军,email:gujun01@medmail.com.cn E-mail:gujun01@medmail.com.cn
  • 基金资助:
    973计划课题分课题(2005CB523105)

Relationship between the expression of multidrug resistant protein genes and fluconazole resistance in clinical isolates of Candida albieans

QIN Xiao-feng1, WU Jian-hua2, HUANG Yi1, JIANG Yuan-ying3, GU Jun2   

  1. Department of Dermatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2006-12-08 Online:2007-08-15 Published:2007-08-15

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摘要： 目的 探讨临床分离白念珠菌氟康唑耐药株/敏感株多药耐药蛋白基因CDR1、CDR2、MDR1的表达情况。方法 试剂盒法抽提白念珠菌氟康唑耐药株/敏感株的总RNA后,逆转录成cDNA,采用实时定量PCR技术观察白念珠菌多药耐药蛋白基因的表达。结果 白念珠菌耐药株组CDR2基因的ΔCT值为7.52±2.53,敏感株组为9.28±3.15,两组比较,t=2.37,P<0.05,耐药株的表达量要高于敏感株。结论 白念珠菌CDR2基因的高表达与氟康唑耐药有关。

关键词: 念珠菌, 白色, 氟康唑, 基因, MDR, 抗药性, 真菌

Abstract: Objective To explore the relationship between fluconazole resistance and expression of multidrug resistant protein genes,including CDR1,CDR2,MDRI.Methods The total RNA was extracted from fluconazole-resistant and-susceptible Candida albicans isolates,and cDNA was synthesized.The expression of CDR1,CDR2 and MDRl genes was then detected by quantitative real-time PCR.TheΔCT (threshold cycle) value was obtained by subtracting the CT value of 18S rRNA from that of the targeted gene.Results TheΔCT value of CDR2 was significantly lower in fluconazole-resistant isolates than in fluconazolesusceptible isolates (7.52±2.53 vs.9.28±3.15,t=2.367,P<0.05),indicating the expression of CDR2 gene was higher in the fluconazole-resistant isolates than in the fluconazole-susceptible isolates.Conclusion It is likely that the over-expression of CDR2 gene is relevant to fluconazole resistance in Candida albicans.

Key words: MDR, Drug resistance, fungal, Candida albicans, Fluconazole, Genes