中华皮肤科杂志

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皮肤念珠菌病患者多部位分离菌株DNA分型研究

佘晓东1, 王学军2, 符美华1, 沈永年1, 刘维达1   

  1. 1. 中国医学科学院皮肤病研究所 南京 210042;
    2. 辽宁省大连市皮肤病医院
  • 收稿日期:2007-01-15 出版日期:2007-08-15 发布日期:2007-08-15
  • 通讯作者: 刘维达,email:liumyco@hotmail.com E-mail:liumyco@hotmail.com

Genotyping of Candida albicans isolated from different body locations of patients with cutaneous candidiasis

SHE Xiao-dong1, WANG Xue-jun2, FU Mei-hua1, SHEN Yong-nian1, LIU Wei-da1   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Nanjing 210042, China
  • Received:2007-01-15 Online:2007-08-15 Published:2007-08-15

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摘要： 目的 比较皮肤念珠菌病患者不同部位分离菌株基因相似性,推测皮肤念珠菌病多部位感染的可能途径。方法 采用PCR扩增引物P-Ⅰ和P-Ⅱ扩增出白念珠菌染色体25SrDNA片段和特征性的基因片段重复序列片段,结合两种扩增结果进行分型,并将870bp大小的特征性的基因片段重复序列片段用限制性内切酶EcoRⅠ和ClaⅠ消化。结果 来自19例皮肤念珠菌病患者的41株白念珠菌被分为6型,同一患者不同部位分离菌株基因型相似,不同个体间基因型有差异。结论 分离自皮肤念珠菌病患者不同部位的致病菌株基因型相同,提示其发病可能与外源性再发感染无关。

关键词: 念珠菌病, 皮肤, DNA指纹法, 感染途径

Abstract: Objective To assess the genotype similarity of Candida albicans isolated from different body locations of patients with cutaneous candidiasis,in order to investigate the possible pathogenesis of cutaneous candidiasis of multiple sites.Methods Specimens were collected for fungal culture.DNA was extracted from the isolates,and amplified by PCR using primers P-Ⅰand P-Ⅱto determine the 25S rDNA and specific repeptive sequence (RPS)-based genotype of the strains.Also,the specific 870 bp-long RPS fragment was digested by restricted endonucleases EcoRⅠand ClaⅠ.Results Forty-one strains of C.albicans were isolated from 19 patients,and classified into 6 genotypes with the above technique.The genotypes of C albicans were identical from different body sites of the same individual,but varied among different patients. Conclusion The genotypes of C.albicans isolates from multiple body locations of a same individual with cutaneous candidiasis are identical,indicating that the pathogenesis of cutaneous candidiasis of multiple sites might be unrelated to an exogenous reinfection with a new species of pathogen.

Key words: Candidiasis, cutaneous, DNA finger printing, Infection route