中华皮肤科杂志

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内皮素受体B在黑素瘤中的表达及内皮素3对黑素瘤细胞A375的体外促生长效应

黄长征1, 田进1, 林能兴1, 陶娟1, 李延1, 刘业强1, 杨凌云1, 张晶1, 古江增隆3, 沈关心2, 李家文1, 王椿森1, 涂亚庭1   

  1. 1. 华中科技大学同济医学院附属协和医院皮肤科, 武汉, 430022;
    2. 华中科技大学同济医学院免疫学系;
    3. 日本九州大学病院皮肤科
  • 收稿日期:2006-10-17 出版日期:2007-07-15 发布日期:2007-07-15
  • 通讯作者: 黄长征,email:hcz0501@126.com E-mail:hcz0501@126.com
  • 基金资助:
    国家自然科学基金(30671891)

Expression of endothelin receptor B in human malignant melanoma and in vitro promoting effect of endothelin 3 on the proliferation of a melanoma cell line A375

HUANG Chang-zheng1, TIAN Jin1, LIN Neng-xing1, TAO Juan1, LI Yan1, LIU Ye-qiang1, YANG Ling-yun1, ZHANG Jing1, Masutaka3, Furuc2, SHEN Guan-xin1, LI Jia-wen1, WANG Chun-sen1   

  1. Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
  • Received:2006-10-17 Online:2007-07-15 Published:2007-07-15

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摘要： 目的 研究内皮素受体B(ETR-B)在人恶性黑素瘤中的表达及内皮素3对黑素瘤细胞A375的体外促生长效应.方法 采用免疫组化SP法检测ETR-B在黑素瘤组织中的表达,用RT-PCR法检测ETR-B基因在人恶性黑素瘤细胞A375和SK-mel-1中的表达,用MTT比色法检测不同浓度内皮素3对黑素瘤细胞A375的体外促增殖活性.结果 ETR-B在41例恶性黑素瘤和23例色素痣中的阳性率分别为78.05%和8.69%,两组间差异有统计学意义.P<0.05.15例原位和26例Ⅰ～Ⅳ期恶性黑素瘤ETR-B阳性表达率分别为53.33%和92.31%,两组比较.P<0.05.13例转移性恶性黑素瘤(Ⅲ～Ⅳ期)中ETR-B阳性表达率100%,28例未转移者(0～Ⅱ期)的阳性表达率为67.86%,两组比较,P<0.05.ETR-B基因在人恶性黑素瘤细胞A375和SK-mel-1中均有表达;内皮素3对黑素瘤细胞A375在体外具有很强的促增殖作用,且促增殖能力呈内皮素3浓度依赖性.结论 内皮素3/ETR-B在促黑素瘤细胞生长中有重要作用.

关键词: 黑色素瘤, 受体, 内皮素B, 内皮缩血管肽3, A375, SK-mel-1

Abstract: Objective To investigate the expression of endothelin receptor B(ETR-B)in human malignant melanoma(MM)and in vitro promoting effect of endothelin 3(ET3)on the proliferation of a melanoma cell line A375.Methods Immunohistochemistry SP method was used to detect the expression of ETR-B in 41 cases of MM and 23 cases of pigmented nevus.RT-PCR was applied to evaluate the mRNA expression of ETR-B gene in melanoma cell lines A375 and SK-mel-1,and MTT method to assess the in vitro effect of ET3 on the proliferation of A375 cells.Results There was a significant difference in the positive rates of ETR-B mRNA expression between 41 cases of MM and 23 cases of pigmented nevus (78.05% vs 8.69%,P<0.05),15 cases of MM in situ and 26 cases ofⅠ-ⅣstageMM(53.33% vs 92.31%,P<0.05),13 cases of metastatic MM at stage Ⅲ-Ⅳ and 28 cases of non metastatic MM at stage 0-Ⅱ(100% vs 67.86%,P<0.05).The ETR-B mRNA was expressed in both A375 and SK-mel-1 cells. ET3 had a strong,concentration-dependent enhancing effect on the proliferation of A375 cells in vitro. Conclusion ET3/ETR-B might have an important role in promoting the proliferation of MM cells.

Key words: Melanoma, Receptor,endothelin B, Endothelin-3, A375, SK-mel-1