中华皮肤科杂志

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DNA甲基转移酶与CD11a基因在SLE患者中的表达

施伟民1, 伍洲炜2, 施若非1, 梅兴宇2, 秦海红2, 郑捷1   

  1. 1. 上海交通大学医学院附属瑞金医院皮肤科, 200065;
    2. 同济大学附属同济医院皮肤科
  • 收稿日期:2006-09-04 出版日期:2007-07-15 发布日期:2007-07-15
  • 通讯作者: 郑捷,email:jie-zheng2001@126.com E-mail:jie-zheng2001@126.com

Expression of DNA methyltransferases and CD11a genes in systemic lupus erythematosus patients

SHI Wei-min1, WU Zhou-wei2, SHI Ruo-fei1, MEI Xing-yu2, QIN Hai-hong2, ZHENG Jie1   

  1. Department of Dermatology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
  • Received:2006-09-04 Online:2007-07-15 Published:2007-07-15

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摘要： 目的 探讨DNA甲基转移酶(DNMT)的表达异常在SLE发病中的作用.方法 以半定量RT-PCR方法检测了SLE缓解期、活动期及正常人外周血单核细胞(PBMC)中DNMT及CD11a基因表达水平,并进行相关性分析.结果 SLE缓解期患者PBMC中DNMT1的表达水平显著低于正常人对照组(t=5.90,P<0.0001);活动期的表达水平也显著低于对照组(t=2.26,P=0.0001);缓解期与活动期比较差异无统计学意义(t=1.75,P=0.089).SLE缓解期、活动期及对照组PBMC中DNMT3A的表达水平差异无统计学意义,DNMT3B的表达水平极低.SLE缓解期PBMC中CD11a表达水平明显高于对照组(t=5.35,P<0.0001);活动期的表达水平显著高于缓解期(t=2.99,P=0.006)和正常人对照组(t=6.57,P<0.0001).DNMT1的降低与SLE疾病活动指数(SLEDAI)间无显著相关性(r=-0.34,P>0.05),CD11a的升高与SLEDAI间呈显著正相关(r=0.48,P<0.05),DNMT1与CD11a间无显著相关性(r=-0.18,P>0.05).结论 DNMT1表达水平降低在SLE的发病中可能起作用.但不是决定DNA甲基化状态的惟一因素.

关键词: 红斑狼疮, 系统性, DNA修饰甲基酶类, 抗原, CD11a

Abstract: Objective To investigate the role of abnormal expression of DNA methyltransferases (DNMT1,DNMT3A,DNMT3B)and CD11a genes in the pathogenesis of systemic lupus erythematosus (SLE).Methods Semiquantitative reverse transcriptase-polymerase chain reaction was applied to detect the mRNA expression levels of DNMTs and CD11a in PBMCs of relieved(n=17),active(n=17)SLE patients and healthy controls(n=17).The correlations were further analyzed between these parameters. Results The expression of DNMT1 was significantly lower in PBMCs of both relieved and active SLE patients than that of health controls(t=5.90,P<0.0001;t=2.26,P=0.0001 respectively).No significant difference was observed in DNMT1 expression between relieved and active SLE patients(t=1.75,P=0.089),or in the expression of DNMT3A in PBMCs between relieved,active SLE patients and healthy control.The expression of DNMT3B in PBMCs was extremely low.The expression of CD11a was significantly higher in relieved SLE patients than in the healthy controls(t=5.35,P<0.0001),in active SLE patients than in relieved SLE patients(t=2.99,P=0.006)and healthy controls(t=6.57,P<0.0001). No significant correlation was observed between the decrease of DNMT1 and SLE disease activity index (SLEDAI)(r=-0.34,P>0.05),or between the expression level of DNMTI and CD11a(r=-0.18, P>0.05).The increase of CD11a positively correlated with SLEDAI(r=0.48,P<0.05).Conclusion The decreased expression of DNMT1 may play an important role in the pathogenesis of SLE,but not the only factor contributing for DNA methylation status.

Key words: Lupus erythematosus,systemic, DNA modification methylases, Antigens,CD11a