中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (8): 551-554.

• 论著 • 上一篇    下一篇

生殖支原体对大环内酯类抗生素耐药突变位点研究

刘排1,蒋娟2,张津萍3,尢永燕4,沙仲4,孙厚华4,龚匡隆2,孙建方2   

  1. 1. 江西省皮肤病专科医院
    2. 南京 中国医学科学院北京协和医学院皮肤病研究所
    3. 中国医学科学院皮肤病研究所
    4. 南京医科院皮研所
  • 收稿日期:2013-11-13 修回日期:2014-04-08 发布日期:2014-08-01
  • 通讯作者: 蒋娟 E-mail:drjjiang@vip.163.com

Detection of point mutations associated with macrolide resistance in Mycoplasma genitalium

  • Received:2013-11-13 Revised:2014-04-08 Published:2014-08-01

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摘要： 【摘要】 目的 了解生殖支原体对大环内酯类抗生素耐药相关的23S rRNA基因突变情况。 方法 就诊于我院性病门诊的91例近期应用过大环内酯类药物治疗的持续性和复发性尿道炎患者，取尿道拭子和前段尿标本进行生殖支原体（Mg）、沙眼衣原体（Ct）、解脲脲原体（Uu）等病原体检测，筛选出单一生殖支原体阳性患者标本，应用套式PCR扩增与大环内酯类抗生素耐药性相关的23S rRNA基因V区片段，扩增产物进行DNA测序，测得序列与美国国立生物信息中心已登记的生殖支原体标准株G37的相应基因序列比对。 结果 91例的标本中，Mg阳性21例，Ct阳性18例（19.8%），Uu阳性10例，Mg与UU同时阳性2例，Ct与Uu同时阳性3例，Mg、Ct和Uu检测均为阴性者37例。21例Mg阳性标本中，18例的标本成功扩增出23S rRNA基因V区片段，除1例未发现基因突变外，其余17例均发现2058和2059位点突变，其中A2059G突变10例，A2058G突变5例，A2058T突变2例。 结论 23S rRNA基因V区片段基因突变可能与南京及周边地区Mg对大环内酯类药物耐药性相关。

关键词: 支原体，生殖器, 抗药性，细菌, 抗菌药

Abstract: Liu Pai, Jiang Juan, Zhang Jinping, You Yongyan, Sha Zhong, Sun Houhua, Gong Kuanglong, Sun Jianfang. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China Corresponding authors: Jiang Juan, Email: drjjiang@vip.163.com; Sun Jianfang, Email: sunjf57@163.com 【Abstract】 Objective To detect point mutations associated with macrolide resistance in the 23S rRNA gene of M. genitalium. Methods Ninty-one patients with persistent or recurrent urethritis, who had been treated with macrolides in the past one month but achieved no obvious improvement, were included in this study. Urethral swab and first-void urine samples were collected from these patients. Gram′s staining, culture and fluorescence-based quantitative PCR were carried out to detect Neisseria gonorrhoeae, Ureaplasma urealyticum and Chlamydia trachomatis respectively in these urethral swab samples, and PCR was performed to detect M. genitalium in the urine samples. For patients positive to only M. genitalium, nested PCR was conducted to amplify the domain V of the 23S rRNA gene followed by direct automatic sequencing, and the resulting sequences were compared to the corresponding sequences of M. genitalium G37 strain submitted to the National Center for Biotechnology Information. Results Of the 91 patients, 21 were positive for M. genitalium, 18 for C. trachomatis, 10 for U. urealyticum, 2 for both M. genitalium and U. urealyticum, 3 for both C. trachomatis and U. urealyticum, and 37 for none of the tested pathogens. The domain V of the 23S rRNA gene, which was amplified from 18 of the 21 M. genitalium-positive samples, carried an A to G transition at position 2059 in 10 samples, an A to G transition at position 2058 in 5 samples, an A to T transition at position 2058 in 2 samples, but no point mutations in 1 sample. Conclusion The point mutations in domain V of the 23S rRNA gene may contribute to macrolide resistance in M. genitalium in Nanjing and its surrounding areas.

Key words: Mycoplasma genitalium, Drug resistance, bacterial, Anti-bacterial agents

引用本文

刘排 蒋娟 张津萍 尢永燕 沙仲 孙厚华 龚匡隆 孙建方. 生殖支原体对大环内酯类抗生素耐药突变位点研究[J]. 中华皮肤科杂志, 2014,47(8):551-554. doi: