中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (11): 796-799.

• 论著 • 上一篇    下一篇

高压氧对兔耳增生性瘢痕形成过程中缺氧诱导因子1α和胰岛素样生长因子Ⅰ受体表达的影响

任纪祯1,陈振雨2,杨廷1,万金娥1,赵明1   

  1. 1. 青岛大学医学院附属医院
    2. 青岛大学医学院附属医院烧伤整形外科
  • 收稿日期:2013-12-05 修回日期:2014-06-16 出版日期:2014-11-15 发布日期:2014-11-01
  • 通讯作者: 陈振雨 E-mail:czy.plastic@126.com
  • 基金资助:
    山东省科技发展计划项目

Effects of hyperbaric oxygen on the expressions of hypoxia-inducible factor 1α and insulin-like growth factor-1 receptor in the formation of hyperplastic scar in rabbit ears

  • Received:2013-12-05 Revised:2014-06-16 Online:2014-11-15 Published:2014-11-01

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摘要： 目的 探讨高压氧对兔耳增生性瘢痕的形成及瘢痕组织中缺氧诱导因子1α（HIF-1α）、胰岛素样生长因子Ⅰ受体（IGF-1R）表达的影响。 方法 选取20只新西兰大白兔建立兔耳瘢痕模型，随机分成实验组和对照组，实验组根据给予高压氧时间不同分为7 d、14 d、21 d及28 d组，每组4只共48个创面，实验组术后立即给予高压氧处理，对照组处于常氧压空气环境中。术后第29天切取瘢痕组织进行苏木精-伊红（HE）染色，观察形态学差异，计算瘢痕增生指数；免疫组化法检测HIF-1α、IGF-1R的表达。 结果 HE染色可见实验组成纤维细胞数量及胶原纤维含量较对照组明显减少。对照组瘢痕增生指数为4.28 ± 0.22，7 d、14 d、21 d及28 d实验组分别为3.64 ± 0.29、3.46 ± 0.21、3.29 ± 0.21、3.16 ± 0.15，各组间差异有统计学意义（F = 77.70，P < 0.05）。免疫组化检测可见，各实验组HIF-1α、IGF-1R的表达量均较对照组显著降低（P < 0．01），14 d组两因子表达量较7 d组、21 d组较14 d组减少（均P < 0．05），28 d组与21 d组比较，差异无统计学意义（均P ＞ 0．05）。 结论 高压氧可降低兔耳瘢痕组织中HIF-1α、IGF-1R的表达，对兔耳增生性瘢痕的形成有明显抑制作用。

关键词: 高压氧, 瘢痕, 缺氧诱导因子1，α亚基, 胰岛素样生长因子-I受体

Abstract: Ren Jizhen, Chen Zhenyu, Yang Ting, Wan Jin′e, Zhao Ming. Affiliated Hospital of Qingdao University, Qingdao 266003, China Corresponding author: Chen Zhenyu, Email: czy.plastic@126.com 【Abstract】 Objective To evaluate the effects of hyperbaric oxygen on the expressions of hypoxia-inducible factor 1α （HIF-1α） and insulin-like growth factor-1 receptor （IGF-1R） in the formation of hyperplastic scar in rabbit ears. Methods The ears of 20 New Zealand rabbits were used to construct an animal model for hyperplastic scar by operation. After the establishment of scar models, the rabbits were randomly divided into 4 experimental groups and one control group with 4 mice （48 wound surfaces） in each group. The mice in the 4 experimental groups were treated with hyperbaric oxygen for 7, 14, 21 and 28 days, respectively, and those in the control group remained in normoxic environment after operation. Scar tissues were resected from all the rabbit ears on day 29 after operation. Hematoxylin and eosin （HE） staining was conducted for the observation of morphological changes and calculation of scar elevation index, and immunohistochemistry to measure the expressions of HIF-1α and IGF-1R. Statistical analysis was carried out by one-way analysis of variance followed by least significant difference t-test. Results HE staining showed that both the number of fibroblasts and amount of collagen fibers were significantly reduced in the experimental groups compared with the control group. Scar elevation index was 4.28 ± 0.22 in the control group, 3.64 ± 0.29, 3.46 ± 0.21, 3.29 ± 0.21, 3.16 ± 0.15 in the 7-, 14-, 21- and 28-day experimental groups respectively, with significant differences among these groups （F = 77.70, P < 0.05）. The expressions of HIF-1α and IGF-1R were significantly lower in these experimental groups than in the control group （all P < 0.01）, lower in the 14-day group than in the 7-day group （P < 0.05）, and lower in the 21-day group than in the 14-day group （P < 0.05）, with no significant differences between the 28-day group and 21-day group （both P > 0.05）. Conclusion Hyperbaric oxygen can effectively down-regulate the expressions of HIF-1α and IGF-1R in scar tissue, and significantly inhibit the formation of hyperplastic scar in rabbit ears.

Key words: Hyperbaric oxygenation, Cicatrix, Hypoxia-inducible factor 1 alpha subunit, Receptor, IGF type 1