中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (3): 196-197.

• 研究报道 • 上一篇    下一篇

白念珠菌耐氟康唑相关基因点突变的检测

景伟芳1,李春莉2,郭雅莉3,刘原君4,亓玉清5,刘全忠6,王惠平2   

  1. 1. 天津医科大学总医院（现在河北廊坊 中国石油中心医院皮肤科 065000）
    2. 天津医科大学总医院皮肤科
    3. 天津医科大学总医院
    4. 天津医科大学总医院/天津性传播疾病研究所
    5. 天津医科大学总医院临床检验科
    6. 天津医科大学总医院皮肤性病科
  • 收稿日期:2012-04-01 修回日期:2012-07-01 出版日期:2013-03-15 发布日期:2013-03-01
  • 通讯作者: 王惠平 E-mail:huiping1208@163.com

Detection of point mutations in genes related to fluconazole resistance in Candida albicans

  • Received:2012-04-01 Revised:2012-07-01 Online:2013-03-15 Published:2013-03-01
  • Contact: Hui-ping WANG E-mail:huiping1208@163.com

PDF

4

赞

摘要： 目的 探讨ERG11、TAC1、MRR1和UPC2点突变与白念珠菌氟康唑耐药之间的关系。 方法 收集耐氟康唑的白念珠菌，尿素裂解法提取DNA，PCR扩增ERG11基因全长以及TAC1、MRR1和UPC2基因的相应片段，送交测序。结果 ERG11基因发现8种点突变，即D116E、Y132H、Y132F、K143Q、T229A、E266D、G448E和G464S，其中K143Q此前未见文献报道。UPC2基因发现1种点突变G648D。TAC1和MRR1基因中均未检测到相应点突变。结论 ERG11点突变与耐药的关系比较明确，TAC1、MRR1和UPC2点突变与耐药的关系有待进一步研究。

关键词: 念珠菌，白色, 药物耐受性, 氟康唑, 点突变

Abstract: JING Wei-fang, LI Chun-li, GUO Ya-li, LIU Yuan-jun, QI Yu-qing, LIU Quan-zhong, WANG Hui-ping*. *Department of Dermatology and Venereology, General Hospital of Tianjin Medical University, Tianjin 300052, China Corresponding author: WANG Hui-ping, Email: huiping1208@163.com 【Abstract】 Objective To investigate the relationship of point mutations in ERG11, TAC1, MRR1 and UPC2 genes to fluconazole resistance in Candida albicans. Methods This study included 11 fluconazole-resistant Candida albicans strains. DNA was extracted from these Candida isolates by urea pyrolysis method. PCR was carried out to amplify the full-length EGR11 gene, as well as the corresponding fragments of TAC1, MRR1 and UPC2 genes followed by DNA sequencing. Results Eight mutations were detected in the ERG11 gene, including D116E, Y132H, Y132F, K143Q, T229A, E266D, G448E and G464S. Among them, K143Q was a novel mutation and had not been reported. A G648D mutation was detected in the UPC2 gene. No mutations were detected in the TAC1 or MRR1 gene. Conclusions There is a definite association between fluconazole resistance and ERG11 mutations, while further research is warranted to clarify the relationship between the mutations of TAC1, MRR1, UPC2 genes and resistance to fluconazole.