中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (10): 707-710.

• 论著 • 上一篇    下一篇

B淋巴细胞刺激因子对慢性特发性荨麻疹患者产生抗FcεRI抗体和抗IgE抗体的影响

康尔恂1,李杰孙丽伟3,韩春玉4,闫丽萍5,杨建   

  • 收稿日期:2013-02-18 修回日期:2013-03-29 出版日期:2013-10-15 发布日期:2013-10-01
  • 通讯作者: 康尔恂 E-mail:kangerxun2000@163.com
  • 基金资助:
    山东省科技攻关项目

Influence of B lymphocyte stimulator on the production of anti-FcεRI and anti-IgE antibodies by B lymphocytes from patients with chronic idiopathic urticaria

  • Received:2013-02-18 Revised:2013-03-29 Online:2013-10-15 Published:2013-10-01

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摘要： 【摘要】 目的 探讨B淋巴细胞刺激因子（BlyS）能否刺激慢性特发性荨麻疹（CIU）患者B淋巴细胞产生抗FcεRI抗体或抗IgE抗体。 方法 设CIU患者组和健康对照组。ELISA法测定血清中BlyS、抗FcεRI抗体和抗IgE抗体水平，分离培养受试者外周血B淋巴细胞，在培养液中加入BlyS，检测培养液中抗FcεRI抗体和抗IgE抗体水平，分析BlyS与抗FcεRI抗体和抗IgE抗体产生的相关性。结果 CIU患者血清BlyS水平显著高于健康对照组（t = 3.04，P < 0.01），抗FcεRI抗体和抗IgE抗体水平均显著高于健康对照组（t = 3.51，P < 0.01；t = 3.29，P < 0.01）。CIU患者血清中抗FcεRI抗体和抗IgE抗体水平与BlyS水平呈正相关（r = 0.93，P < 0.01；r = 0.91，P < 0.01）；CIU患者外周血B淋巴细胞培养液中加入有效浓度BlyS后，B淋巴细胞培养液中抗FcεRI抗体和抗IgE抗体水平均显著高于不加BlyS的空白对照 （t = 3.67，P < 0.01；t = 3.56，P < 0.01），2种抗体在培养液中的水平与BlyS浓度呈正相关（r = 0.96，P < 0.01；r = 0.91，P < 0.01）；抗FcεRI抗体和抗IgE抗体在CIU患者血清与培养液中的检出符合率分别为94.76%和87.84%。 结论 CIU患者血液中BlyS水平增高可刺激B淋巴细胞产生抗FcεRI抗体或抗IgE抗体，可能与CIU发病有关。 【关键词】 荨麻疹； B淋巴细胞刺激因子； 抗体，FcεRI； 抗体，IgE； B淋巴细胞

关键词: 荨麻疹, B淋巴细胞刺激因子, 抗体，FcεRI, 抗体，IgE, B淋巴细胞

Abstract: KANG Er-xun*, LI Jie, SUN Li-wei, HAN Chun-yu, YAN Li-ping, YANG Jian. *Department of Dermatology, Affiliated Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai 264000, Shandong, China Corresponding author: KANG Er-xun, Email: kangerxun2000@163.com 【Abstract】 Objective To explore if B lymphocyte stimulator （BlyS） stimulates B lymphocytes from patients with chronic idiopathic urticaria （CIU） to produce anti-high affinity IgE receptor （FcεRI） or anti-IgE antibodies. Methods Totally, 300 CIU patients and 300 health controls were enrolled in this study. Blood samples were obtained from these subjects. Peripheral blood B lymphocytes were isolated and cultured in vitro for 72 hours. Then, BlyS of various concentrations （2, 4, 8, 16 ng/ml） was added to the culture medium of B lymphocytes followed by another 72-hour culture. Enzyme-linked immunosorbent assay was performed to determine the serum levels of BlyS, anti-FcεRI and anti-IgE antibodies, as well as the supernatant levels of anti-FcεRI and anti-IgE antibodies. The relationship between BlyS and anti-FcεRI and anti-IgE antibody production was assessed. SPSS software version 16.0 was used for statistical analysis. Chi-square test was performed to compare the positivity rate of antibodies, and analysis of variance and least significance difference-t test to assess numerical data. Results The CIU patients showed higher levels of serum BlyS（t = 3.04, P < 0.01）, anti-FcεRI antibodies（t = 3.51, P < 0.01）, and anti-IgE antibodies （t = 3.29, P < 0.01） compared with the health controls. The serum level of BlyS was positively correlated with that of anti-FcεRI antibodies （r = 0.93, P < 0.01） and anti-IgE antibodies （r = 0.91, P < 0.01）. The levels of anti-FcεRI antibodies and anti-IgE antibodies were significantly increased in the culture supernatant of patient-derived B lymphocytes treated with BlyS compared with those remaining untreated （t = 3.67, 3.56, respectively, both P < 0.01）, and the concentration of BlyS was positively correlated with the levels of both anti-FcεRI antibodies and anti-IgE antibodies（r = 0.96, 0.91, respectively, both P < 0.01）. The coincidence rate between the serum and supernatant was 94.76% and 87.84% in the detection of anti-FcεRI antibodies and anti-IgE antibodies respectively. Conclusions BlyS level is upregulated in the serum of patients with CIU, which may play an important role in the pathogenesis of CIU by stimulating B lymphocytes to produce anti-FcεRI antibodies or anti-IgE antibodies. 【Key words】 Urticaria; B cell activating factor; Antibodies, FcεRI; Antibodies, IgE; B-lymphocytes

Key words: B lymphocyte stimulator

中图分类号: 

  • R739.5