中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (1): 58-60.

• 研究报道 • 上一篇    下一篇

云南马齿苋提取物的抗炎机制研究

庞勤1,邹菥2,何黎3   

  1. 1. 昆明薇诺娜皮肤医疗美容门诊部
    2. 昆明医学院第一附属医院皮肤科
    3. 昆明市昆明医学院第一附属医院皮肤科
  • 收稿日期:2012-03-23 修回日期:2012-04-10 出版日期:2013-01-15 发布日期:2013-01-01
  • 通讯作者: 何黎 E-mail:drheli2662@126.com
  • 基金资助:
    云南省省院省校合作项目

Anti-inflammatory mechanisms of purslane from Yunnan province: an experimental study

1,   

  • Received:2012-03-23 Revised:2012-04-10 Online:2013-01-15 Published:2013-01-01

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摘要： 目的 探讨马齿苋提取物对肿瘤坏死因子?琢（TNF-?琢）、细胞间黏附分子1（ICAM-1）表达的影响，初步阐明马齿苋抗炎机制。方法　建立二甲苯致小鼠耳廓肿胀炎症模型，并分为4组。空白组：不采用任何干预措施，阴性对照组：建立模型后，不加干预，阳性组：模型建立后，使用韩国进口马齿苋外涂小鼠致炎处，实验组：建立模型后，使用云南马齿苋提取物涂于致炎处。组织病理及免疫组化观察TNF-?琢、ICAM-1的变化并比较各组的抗炎效果。结果　组织病理：空白组：真皮可见血管及少量淋巴细胞；阴性对照组：真皮组织疏松、水肿及大量淋巴细胞浸润；阳性组：轻度水肿，真皮血管周围可见部分炎症细胞浸润；实验组：HE染色：真皮轻度水肿，血管周围可见部分炎症细胞浸润。免疫组化：空白组：TNF-?琢、ICAM-1未见表达；阴性对照组：TNF-?琢强阳性表达于血管内皮细胞膜，ICAM-1强阳性表达于血管内皮细胞膜及淋巴细胞膜；阳性组：TNF-?琢阳性表达于血管内皮细胞膜，ICAM-1阳性表达于血管内皮细胞膜及淋巴细胞膜；实验组： TNF-?琢阳性表达于血管内皮细胞膜，ICAM-1阳性表达于血管内皮细胞膜及淋巴细胞膜。秩和检验：实验组与空白组比较TNF-?琢、ICAM-1，P值均 < 0.01，差异有统计学意义。结论　云南马齿苋抗炎机制可能与影响TNF-?琢、ICAM-1的表达有关。

关键词: 马齿苋, 抗炎剂（中药）

Abstract: PANG Qin, ZOU Xi, HE Li*. *Department of Dermatology, First Affiliated Hospital of Kunming Medical University, Yunnan Research Center for Dermatology and Venereology, Kunming 650032, China Corresponding author: HE Li, Email: heli2662@yahoo.com.cm 【Abstract】 Objective To explore the anti-inflammatory mechanisms of purslane by evaluating its effects on the expression of tumor necrosis factor-α （TNF-α） and intercellular adhesion molecule 1 （ICAM-1）. Methods A model of inflammation was developed in 45 mice by painting xylene to the auricle of the right ears, which were then divided into 3 groups to receive no treatment （negative control group）, be topically treated with the extraction of purslane from South Korea （positive control group） or Yunnan province （experimental group）. Fifteen mice receiving no sensitization nor treatment served as the blank control group. Two hours after the single topical treatment, skin tissue samples were obtained from the site of experimental inflammation and subjected to pathological examination by using hematoxylin and eosin （HE） staining. Immunohistochemistry was performed to quantify the expression of TNF-α and ICAM-1 in the tissue samples. Results Pathological examination showed blood vessels and a small quantity of lymphocytes in murine dermis of the blank control group as well as loose and edematous dermis infiltrated with massive lymphocytes in the negative control group. However, there was only mild edema and perivascular infiltration with some inflammatory cells in the dermis of the positive control group and experimental group. Neither TNF-α nor ICAM-1 was expressed in the skin tissue of the blank control group, but an intense expression was observed for TNF-α in the vascular endothelial cell membrane and for ICAM-1 in the vascular endothelial cell membrane and lymphocyte membrane in the negative control group, which was significantly downregulated by the purslane from South Korea in the positive control group and by the purslane from Yunnan province in the experimental group （all P < 0.01） . Rank sum test showed a statistical difference in the expression level of TNF-α and ICAM-1 between the blank control group and experimental group （both P < 0.01）. Conclusion The purslane from Yunnan province may counteract inflammation by affecting the expression of TNF-α and ICAM-1.