中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (4): 249-251.

• 论著 • 上一篇    下一篇

白癜风患者外周血单一核细胞Toll样受体7、9 mRNA表达的初步研究

唐亚平,李振洁,林春生,黎小东   

  1. 广州市皮肤病防治所
  • 收稿日期:2011-06-27 修回日期:2011-09-26 出版日期:2012-04-15 发布日期:2012-03-30
  • 通讯作者: 唐亚平 E-mail:tangya1130@126.com
  • 基金资助:

    广州市医药卫生科技项目

Expressions of Toll-like receptor 7 and 9 mRNA in peripheral blood mononuclear cells from patients with vitiligo

  • Received:2011-06-27 Revised:2011-09-26 Online:2012-04-15 Published:2012-03-30

PDF

21

赞

摘要：

目的 探讨Toll样受体7、9 （TLR7、TLR9）mRNA在白癜风患者外周血单一核细胞（PBMC）中的表达及其意义。方法 应用实时荧光定量逆转录聚合酶链反应（RT-PCR）技术检测50例白癜风患者及25例正常人对照PBMC中TLR7、TLR9 mRNA的表达。采用SPSS11.5软件进行统计学分析，两样本均数比较采用t检验。结果 白癜风患者PBMC中TLR7 mRNA（目的基因绝对定量校正拷贝数为0.85 ± 1.90）、TLR9 mRNA（0.94 ± 2.25）表达均明显高于对照组（分别为0.44 ± 1.18和0.11 ± 0.31，P ＜ 0.05和 ＜ 0.01）；稳定期与进展期、局限型与泛发型白癜风患者TLR7、TLR9 mRNA表达差异均无统计学意义（P值均 ＞ 0.05）。结论 白癜风患者PBMC中TLR7、TLR9 mRNA的表达均上调，TLR7、TLR9的异常表达可能参与白癜风的发病机制。

关键词: RT-PCR

Abstract:

Objective To detect the expressions of Toll-like receptor （TLR） 7 and 9 mRNA in peripheral blood mononuclear cells （PBMCs） from patients with vitiligo and their significance. Methods Real-time fluorescence-based quantitative reverse transcription-PCR was performed to detect the expressions of TLR7 and TLR9 mRNA in PBMCs from 50 patients with vitiligo and 25 normal human controls. Two-sample t test was conducted by using SPSS 11.5 software to compare the expression difference of TLR7 and TLR9 mRNA between the patients and controls. Results The mRNA expression level （the ratio of the absolute copy number of a target gene to a control gene） of TLR7 and TLR9 in PBMCs were significantly higher in patients with vitiligo than in the normal human controls （0.85 ± 1.90 vs. 0.44 ± 1.18, P ＜ 0.05; 0.94 ± 2.25 vs. 0.11 ± 0.31, P ＜ 0.01）. No significant difference was observed in the expression level of TLR7 or TLR9 mRNA in PBMCs between patients with stable and active vitiligo, or between patients with localized and generalized vitiligo （both P > 0.05）. Conclusions The expression level of TLR7 and TLR9 mRNA is elevated in PBMCs from patients with vitiligo, which may be involved in the pathogenesis of vitiligo.

Key words: RT-PCR