中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (4): 252-254.

• 论著 • 上一篇    下一篇

特应性皮炎患儿外周血转录因子RORγt与Foxp3的表达

邱盼盼,王忠永   

  1. 山东省滨州医学院附属医院皮肤科
  • 收稿日期:2011-07-02 修回日期:2011-12-30 出版日期:2012-04-15 发布日期:2012-03-30
  • 通讯作者: 王忠永 E-mail:byzhongyong@sohu.com

Expressions of RORγt and Foxp3 in peripheral blood of children with atopic dermatitis

  • Received:2011-07-02 Revised:2011-12-30 Online:2012-04-15 Published:2012-03-30

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摘要：

目的 了解特应性皮炎患儿外周血单一核细胞RORγt基因和Foxp3基因的表达水平，探讨其在特应性皮炎发展进程中的作用。方法 特应性皮炎患儿63例，正常儿童54例作为正常对照组。荧光实时定量PCR检测外周血单一核细胞RORγt基因和Foxp3基因的表达情况。结果 特应性皮炎患儿外周血Foxp3基因和RORγt基因的相对表达水平（分别为2.4060 ± 0.3355和6.9130 ± 0.2046）均显著高于正常对照组（分别为1.1852 ± 0.4189和2.7501 ± 0.2518），两组比较，t值分别为17.50和98.63，P值均 ＜ 0.01。外周血RORγt水平特应性皮炎患儿重度期（7.1203 ± 0.1056）明显高于轻度期（6.8046 ± 0.1731，t = 6.61，P ＜ 0.01）和中度期（6.8655 ± 0.3671，t = 5.23，P ＜ 0.01），而轻度期与中度期比较，差异无统计学意义。Foxp3在轻中重各组间差异均无统计学意义。RORγt与特应性皮炎疾病严重程度呈正相关（r = 0.62，P ＜ 0.01），而Foxp3与疾病严重程度无显著相关性（r = 0.04，P ＞ 0.05）。结论 特应性皮炎的发生与RORγt基因和Foxp3基因表达的升高有关，且RORγt与疾病严重程度呈正相关。

关键词: Foxp3基因

Abstract:

Objective To detect the mRNA expression levels of RORγt and Foxp3 genes in peripheral blood mononuclear cells（PBMCs） of children with atopic dermatitis（AD）, and to explore their possible roles in the development of AD. Methods Sixty-three children with AD and 54 age-matched normal children were eligible for this study. Real-time fluorescence-based quantitative PCR was carried out to measure the mRNA expressions of RORγt and Foxp3 genes in PBMCs of these subjects． Results The relative expression levels of Foxp3 and RORγt mRNA were significantly higher in children with AD compared with the normal controls （2.4060 ± 0.3355 vs. 1.1852 ± 0.4189, t = 17.50, P ＜ 0.01; 6.9130 ± 0.2046 vs. 2.7501 ± 0.2518, t = 98.63, P ＜ 0.01）. A statistical increase was observed in the level of RORγt mRNA in children with severe AD compared with children with mild and moderate AD（7.1203 ± 0.1056 vs. 6.8046 ± 0.1731 and 6.8655 ± 0.3671, t = 6.61, 5.23, both P ＜ 0.01）. No significant difference was noted in the expression of RORγt mRNA between children with mild and moderate AD, or in the Foxp3 mRNA expression among children with mild, moderate and severe AD. The severity of AD was positively correlated with the level of RORγt mRNA（r = 0.62, P ＜ 0.01）, but uncorrelated with the level of Foxp3 mRNA（r = 0.04, P > 0.05）. Conclusions The high expressions of RORγt and Foxp3 genes may be involved in the pathogenesis of AD, and the severity of AD is positively correlated with the expression intensity of RORγt gene．

Key words: Foxp3 gene