中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (3): 155-157.

• 论著 • 上一篇    下一篇

天疱疮和大疱性类天疱疮患者外周血滤泡辅助性T细胞水平的研究

李秋菊1,党二乐1,呼蕾2,吴岩1,刘振锋1,何争1,晋亮1,王刚3   

  1. 1. 第四军医大学西京医院全军皮肤病研究所
    2.
    3. 第四军医大学西京医院皮肤科
  • 收稿日期:2011-06-30 修回日期:2011-07-28 出版日期:2012-03-15 发布日期:2012-02-29
  • 通讯作者: 王刚 E-mail:xjwgang@fmmu.edu.cn

Follicular helper T cells in peripheral blood of patients with pemphigus and bullous pemphigoid

  • Received:2011-06-30 Revised:2011-07-28 Online:2012-03-15 Published:2012-02-29
  • Contact: WANG Gang E-mail:xjwgang@fmmu.edu.cn

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摘要：

目的 探讨滤泡辅助性T细胞（Tfh）在天疱疮和大疱性类天疱疮发病中的作用。 方法 天疱疮和大疱性类天疱疮患者各10例，与天疱疮和大疱性类天疱疮患者年龄、性别相匹配的正常人对照各10例。采用流式细胞仪检测外周血单一核细胞（PBMC）中Tfh水平，采用酶联免疫吸附法（ELISA）检测其血清中白介素21（IL-21）水平，同时检测大疱性类天疱疮患者血清中抗BP180-NC16A抗体滴度，并进行抗体滴度与IL-21、Tfh水平的相关性分析。结果 天疱疮患者组血清中IL-21水平（95.33 ± 33.69 ng/L）显著高于其正常人对照组（54.50 ± 18.13 ng/L）（t = 3.38，P ＜ 0.01），Tfh水平（12.08% ± 4.74%）亦显著高于其正常人对照组（6.15% ± 1.62%）（t = 3.74，P ＜ 0.01）。大疱性类天疱疮患者组血清中IL-21水平（106.70 ± 44.91 ng/L）显著高于其正常人对照组（55.37 ± 15.89 ng/L）（t = 3.41，P ＜ 0.01），Tfh水平（11.85% ± 3.14%）亦显著高于其正常人对照组（6.03% ± 1.74%）（t = 5.13，P ＜ 0.01）。大疱性类天疱疮组抗BP180-NC16A抗体滴度与IL-21水平呈正相关（r = 0.77，P ＜ 0.01），与Tfh水平亦呈正相关（r = 0.67，P ＜ 0.05）。结论 天疱疮和大疱性类天疱疮患者Tfh及其分泌的细胞因子IL-21水平均明显升高，可能在其发病过程中起一定的作用。

关键词: 自身抗体

Abstract:

Objective To explore the roles of follicular helper T cells（Tfh） in the pathogenesis of pemphigus and bullous pemphigoid （BP）. Methods Venous blood was collected from 20 patients （including 10 cases of pemphigus and 10 cases of BP） and 20 age- and sex-matched normal human controls. Enzyme linked immunosorbent assay （ELISA） was performed to measure the levels of serum interleukin （IL）-21 and anti-BP180-NC16A antibody, and flow cytometry to detect the percentage of Tfh in peripheral blood mononuclear cells （PBMCs）. The relationship of anti-BP180-NC16A antibody titer to Tfh and IL-21 levels was assessed by linear correlation analysis. Results The serum IL-21 level and Tfh percentage in PBMCs were significantly higher in patients with pemphigus than in the normal controls （95.33 ± 33.69 vs. 54.50 ± 18.13 pg/ml, t = 3.38, P < 0.01; 12.08% ± 4.74% vs. 6.15% ± 1.62%, t = 3.74, P < 0.01）, and higher in patients with BP than in the normal controls（106.70 ± 44.91 vs. 55.37 ± 15.89 pg/ml, t = 3.41, P < 0.01; 11.85% ± 3.14% vs. 6.03% ± 1.74%, t = 5.13, P < 0.01）. The titer of anti-BP180-NC16A antibody was positively correlated with the percentage of Tfh （r = 0.67, P < 0.05） and serum level of IL-21 （r = 0.77, P < 0.01） in patients with BP. Conclusions There is a significant increase in the percentage of Tfh and serum level of IL-21 in patients with pemphigus and BP, hinting a certain role of Tfh and IL-21 in the pathogenesis of pemphigus and BP.

Key words: autoantibody