中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (12): 859-861.

• 论著 • 上一篇    下一篇

扁平苔藓皮损中钙激活蛋白酶Ⅰ和半胱氨酸天冬氨酸蛋白酶3的表达及其与细胞凋亡的关系

谢欣1,盖希余1,秦晓娟2,张琳1,柴立1   

  1. 1. 黑龙江省皮肤病防治研究所
    2. 哈尔滨市黑龙江省皮肤病防治研究所
  • 收稿日期:2012-02-06 修回日期:2012-03-29 出版日期:2012-12-15 发布日期:2012-11-30
  • 通讯作者: 柴立 E-mail:chaili2221@163.com
  • 基金资助:

    黑龙江省卫生厅科研基金

Expressions of calpain 1 and caspase-3 in lichen planus lesions and their relationship with apoptosis in keratinocytes

  • Received:2012-02-06 Revised:2012-03-29 Online:2012-12-15 Published:2012-11-30

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摘要：

目的 探讨凋亡相关蛋白钙激活蛋白酶Ⅰ（Calpain Ⅰ）和半胱氨酸天冬氨酸蛋白酶3（Caspase-3）在扁平苔藓皮损中的表达及其与细胞凋亡的关系。方法 分别采用脱氧核糖核苷酸末端转移酶介导的原位缺口末端标记（TUNEL）法和免疫组化法对20例扁平苔藓患者皮损组织和10例健康人皮肤组织中细胞凋亡和Calpain Ⅰ、Caspase-3表达进行检测。采用SPSS 13. 0软件，两组凋亡指数比较采用t检验，两组Calpain Ⅰ和Caspase-3表达量比较采用秩和检验，Calpain Ⅰ和Caspase-3表达量与凋亡指数相关性分析采用 Spearman 等级相关分析。结果 扁平苔藓组表皮层角质形成细胞的凋亡指数（67.59 ± 13.50）显著高于健康对照组（28.26 ± 7.56），两组比较，差异有统计学意义（t = 8.52，P < 0.01）；Calpain Ⅰ和Caspase-3表达均高于健康对照组（T = 78.00 和77.00，P < 0.01）。扁平苔藓皮损中Calpain Ⅰ和Caspase-3阳性表达强度均与其自身角质形成细胞的凋亡指数呈正相关（r = 0.71和0.74，P < 0.01）。结论 扁平苔藓皮损中Calpain Ⅰ和Caspase-3表达上调，且与角质形成细胞凋亡亢进关系密切。

关键词: 半胱氨酸天冬氨酸蛋白酶3

Abstract:

Objective To quantify the expression levels of calpain 1 and caspase-3 in lichen planus （LP） lesions and their relationship with the apoptosis in keratinocytes. Methods Biopsy samples were obtained from the lesions of 20 patients with LP and normal skin of 10 healthy controls, and embedded in paraffin. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling （TUNEL） was used to evaluate the apoptosis in keratinocytes, and immunohistochemical staining to detect the expressions of calpain 1 and caspase-3, in these tissue specimens. Data were processed by SPSS 13.0 software. Comparison analysis was carried out by t test for apoptosis index, and by rank sum test for the expressions of calpain 1 and caspase-3. Spearman correlation analysis was conducted to evaluate the relationship between these parameters. Results The apoptosis index of keratinocytes was higher in LP lesions than in the normal skin （67.59 ± 13.50 vs. 28.26 ± 7.56, t = 8.52, P < 0.01）. Significantly increased expressions of calpain 1 and caspase-3 were observed in the epidermis of LP lesions compared with the normal skin （T = 78.00 and 77.00, respectively, both P < 0.01）. The expressions of both calpain 1 and caspase-3 were positively correlated with apoptosis index of keratinocytes （r = 0.71 and 0.74, respectively, both P < 0.01）. Conclusions The expressions of calpain 1 and caspase-3 are upregulated in LP lesions, which may be closely associated with the accelerated apoptosis in keratinocytes.

Key words: Caspase-3