中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (11): 774-777.

• 论著 • 上一篇    下一篇

白介素12B基因rs6887695多态性与汉族人寻常性银屑病临床表型的相关性

陈颖炜1,邬玉美2,薛峰3,鲁智勇4,郑捷5   

  1. 1. 上海瑞金医院卢湾分院
    2. 上海瑞金医院
    3. 上海交通大学医学院瑞金医院皮肤科
    4. 上海交通大学医学院附属新华医院皮肤科
    5. 上海交通大学医学院附属瑞金医院皮肤科
  • 收稿日期:2011-12-14 修回日期:2012-05-20 出版日期:2012-11-15 发布日期:2012-10-31
  • 通讯作者: 陈颖炜 E-mail:chenyingwei2046@126.com

Correlation of interleukin?鄄12B single nucleotide polymorphism rs6887695 with clinical phenotypes of psoriasis vulgaris in Chinese Han population

  • Received:2011-12-14 Revised:2012-05-20 Online:2012-11-15 Published:2012-10-31
  • Contact: Ying-Wei CHEN E-mail:chenyingwei2046@126.com

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摘要：

目的 探讨白介素12B（IL-12B）基因多态性位点rs6887695与汉族人寻常性银屑病临床表型（发病年龄、家族史、临床类型、性别）的相关性。 方法　采用ABI Taqman探针荧光PCR技术，对575例寻常性银屑病患者和1403例健康对照的DNA样本进行IL-12B基因多态位点rs6887695的基因分型。使用SPSS14.0分析软件，χ2检验比较患者组和健康对照组间、不同临床表型组间的基因型和等位基因频率分布的差异性。 结果 IL-12B基因多态性位点rs6887695三种基因型（GG、GC、CC）频率在寻常性银屑病患者组分别为42.61%、45.39%和12.0%，健康对照组分别为34.42%、47.83%和17.75%；等位基因频率（G、C）患者组分别为65.30%和34.70%，健康对照组分别为58.34%和41.66%，基因型和等位基因频率分布在患者组和健康对照组间差异均有统计学意义（χ2值分别为16.31和16.54，P值均 < 0.01），在慢性斑块状（543例）和急性滴状银屑病患者（32例）组间的差异均有统计学意义（χ2值分别为18.11和12.19，P值均 < 0.01）。等位基因G和基因型GG在患者组中的频率明显高于健康对照组，等位基因G和基因型GG在斑块状患者中的频率高于滴状患者。少儿发病组（35例）与成人发病组（540例）、家族史阳性组（102例）与家族史阴性组（440例）、男性患者组（341例）与女性患者组（234例）的基因型和等位基因频率分布差异均无统计学意义（P值均 > 0.05）。 结论 IL-12B（rs6887695）基因多态性与汉族人寻常性银屑病易感性相关联，特别是与斑块状银屑病相关，但与患者的发病年龄、家族史及性别可能无关联。

关键词: 银屑病，临床表型，多态性，IL-12B

Abstract:

Objective To analyze the correlation of interleukin （IL）-12B gene single nucleotide polymorphism （SNP） rs6887695 with clinical phenotypes （including age at onset, family history, clinical types, gender） of psoriasis vulgaris in Chinese Han population． Methods This study recruited 575 patients with psoriasis vulgaris and 1403 healthy controls. DNA samples were obtained from these subjects. PCR with Taqman fluorescent probe （ABI 7900 system） was performed to analyze the genotype of SNP rs6887695 in IL-12B gene. Statistical analysis was carried out by using the software SPSS 14.0, and Chi-square test was conducted to compare the frequency of the SNP rs6887695 genotypes and alleles between the patients and controls as well as between patients with different clinical phenotypes of psoriasis． Results The frequency of GG, GC and CC genotype of the SNP rs6887695 was 42.61%, 45.39% and 12.0% respectively in the patients, compared to 34.42%, 47.83% and 17.75% in the healthy controls （χ2 = 16.31, P < 0.01）; the frequency of G and C allele of the SNP rs6887695 was 65.30% and 34.70% respectively in the patients, compared to 58.34% and 41.66% respectively in the healthy controls （χ2 = 16.54, P<0.01）. Significant differences were observed in the distribution of genotypes and alleles of the SNP rs6887695 between patients with chronic plaque psoriasis （n = 543） and those with acute guttate psoriasis （n = 32, χ2 = 18.11, 12.19, both P < 0.01）. Increased frequency of G allele and GG genotype of the SNP rs6887695 were noted in the patients with psoriasis vulgaris compared with the healthy controls, and in the patients with plaque psoriasis compared with those with guttate psoriasis. However, there was no statistical difference in the distribution of SNP rs6887695 genotypes or alleles between 540 patients with adult onset psoriasis and 35 patients with child onset psoriasis, between 102 patients with family history and 440 patients without family history, or between 341 male patients and 234 female patients （all P > 0.05）. Conclusions The IL-12B SNP rs6887695 may be associated with the susceptibility to psoriasis vulgaris in Chinese Han population, especially with the susceptibility to plaque psoriasis, but seems unassociated with the age at onset, family history or gender of patients.

Key words: psoriasis, clinical phenotype, polymorphism，IL-12B