中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (11): 778-781.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者全血基因组中白介素2共同受体γc甲基化水平检测

马乐1,李亚萍2,康湛苑1,丁漱1,赵明3,黄威1,高飞4,汤媚妮1,程文静1,陆前进5   

  1. 1. 中南大学
    2. 中南大学湘雅二医院皮肤科
    3.
    4. 长沙 中南大学湘雅二医院
    5. 长沙中南大学湘雅二医院皮肤科
  • 收稿日期:2011-12-31 修回日期:2012-03-08 出版日期:2012-11-15 发布日期:2012-10-31
  • 通讯作者: 陆前进 E-mail:qianlu5860@gmail.com
  • 基金资助:

    973重大研究计划

Determination of methylation level of interleukin-2 common receptor gamma chain in the whole blood of patients with systemic lupus erythematosus

  • Received:2011-12-31 Revised:2012-03-08 Online:2012-11-15 Published:2012-10-31

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摘要：

目的 探讨系统性红斑狼疮（SLE）患者外周全血基因组中DNA甲基化生物标记，用于SLE的病情评估。方法 58例SLE患者中重度活动14例，中度活动25例，非活动19例；健康对照组50例，亚硫酸氢盐测序法测定白介素2共同受体?酌c（IL-2RG）启动子区域甲基化水平，实时测定IL-2RG mRNA的表达水平。结果 ①SLE重度活动组、中度活动组、非活动组和健康对照组外周全血IL-2RG启动子区域甲基化水平分别为0.217 ± 0.14０、0.342 ± 0.085、0.325 ± 0.230、0.175 ± 0.036，非活动组高于活动组和健康对照组（P < 0.01），差异有统计学意义。IL-2RG启动子区域甲基化水平SLE患者组（0.283 ± 0.073）高于健康对照组（P < 0.05），差异有统计学意义。②SLE患者组和健康对照组外周血IL-2RG mRNA表达水平分别为2.550 ± 0.823和4.293 ± 1.283，健康对照组高于SLE患者组（P < 0.05），差异有统计学意义。20例SLE患者IL-2RG mRNA表达水平与IL-2RG启动子区域甲基化水平呈负相关（r = -0.44，P < 0.05）。结论 SLE患者全血基因组IL-2RG启动子区域特定CpG位点甲基化水平较健康对照组明显升高，且活动期SLE患者组与稳定期SLE患者组相比全血基因组IL-2RG启动子区域甲基化水平也有差异。

关键词: IL-2RG

Abstract:

Objective To investigate DNA methylation markers in the whole blood of patients with systemic lupus erythematosus （SLE）, in hope to facilitate the evaluation of SLE severity. Methods Whole blood samples were obtained from 58 patients with SLE （including 14 cases of severe SLE, 25 moderate SLE, 19 inactive SLE） and 50 healthy controls. Bisulphite sequencing was performed to determine the methylation status of interleukin-2 common receptor gamma chain （IL-2RG） promoter region, and real-time reverse transcription-PCR to quantify the expression level of IL-2RG mRNA, in these subjects. Results The methylation level of IL-2RG promoter region was 0.217 ± 0.140, 0.325 ± 0.230, 0.342 ± 0.085 and 0.175 ± 0.036 in the patients with severe, moderate and inactive SLE and healthy controls, respectively. A significant increase was observed in the methylation level of IL-2RG promoter region in the patients with inactive SLE compared with the patients with severe SLE and healthy controls （both P < 0.01）, and in the patients with SLE compared with the healthy controls （0.263 ± 0.047 vs. 0.175 ± 0.036, P < 0.05）. The expression level of IL-2RG mRNA was significantly lower in the patients with SLE than in the healthy controls （2.550 ± 0.823 vs. 4.293 ± 1.283, P < 0.05）. A negative correlation was observed between the expression level of IL-2RG mRNA and methylation level of IL-2RG promoter region in 20 patients with SLE （r = －0.44, P < 0.05）. Conclusion The methylation status of IL-2RG promoter region is statistically higher in patients with SLE than in healthy controls, and significantly different between patients with active SLE and those with stable SLE.

Key words: IL-2RG