中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (8): 540-543.

• 论著 • 上一篇    下一篇

Oligo芯片技术分析阴道念珠菌病患者阴道分泌物中致病相关因子的表达谱

佘晓东1,韩峻松2,沈永年1,吕雪莲3,肖华胜4,刘维达1   

  1. 1. 南京 中国医学科学院北京协和医学院皮肤病研究所
    2. 生物芯片上海国家工程研究中心
    3. 大连市皮肤病医院
    4.
  • 收稿日期:2011-01-19 修回日期:2011-04-05 出版日期:2011-08-15 发布日期:2011-07-26
  • 通讯作者: 刘维达 E-mail:liumyco@hotmail.com
  • 基金资助:

    国家自然科学基金;江苏省自然科学基金

Gene expression profiling of pathogenic factors in vaginal secretions of patients with vulvovaginal candidiasis by using Oligo chips

  • Received:2011-01-19 Revised:2011-04-05 Online:2011-08-15 Published:2011-07-26

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摘要：

目的 借助Oligo基因芯片的优势，整体地分析探讨阴道念珠菌病患者阴道分泌物中各致病相关因子的表达。方法 分别提取10例阴道念珠菌病患者及3例无症状带菌者阴道分泌物标本的RNA，将其与疾病相关因子Oligo表达谱芯片杂交，筛选出表达改变超过2倍（比值≥2或≤0.5）的因子，并绘制成差异基因表达谱进行分析。结果 与正常人对照组相比，在患者组中表达上调的基因有44个，其中，MIP-1α、NF-κB、TNF-α、IFN-γ、TLR4、HWP1、SAP2、SAP5、LIP4、EFG1、CPH1在80%以上的标本中表达上调（平均比值为4.013）；表达下调的基因有17个，其中，LIP6、WH11在80%以上的标本中表达下调（平均比值为0.326）。分析表达差异明显因子的生物信息功能，MIP-1α、NF-κB、TNF-α、IFN-γ、TLR4与机体天然免疫相关，HWP1与菌丝黏附及形成相关，SAP2、SAP5、LIP4、LIP6与菌株胞外水解酶相关，EFG1、CPH1、WH11与菌株表型转换有关。结论 宿主适应性免疫功能受限以及致病菌株毒力增强均参与阴道念珠菌病的发病机制，TLR4在该病局部宿主免疫机制中可能起一定作用。

关键词: 发病机制

Abstract:

Objective To analyze the gene expression of pathogenic factors in vaginal secretions of patients with vulvovaginal candidiasis by using Oligo chips. Methods RNA was extracted from vaginal secretions of 10 patients with vulvovaginal candidiasis and 3 asymptomatic carriers, and hybridized with oligonucleotide gene chips. Those genes whose expression was changed by two or more folds （ratio≧2 or ≦0.5） were screened followed by a bioinformatic analysis. Results Comparing with the asymptomatic carriers, the patients showed a higher expression of 44 genes and lower expression of 17 genes. Of these differentially expressed genes, MIP-1α, nuclear factor （NF）-κB, tumor necrosis factor （TNF）-α, interferon （IFN）-γ, Toll like receptor （TLR） 4, HWP1, SAP2, SAP5, LIP4, EFG1 and CPH1 were highly expressed in more than 80% of the secretion samples from patients with an average ratio of 4.013, while LIP6 and WH11 were lowly expressed in more than 80% samples with an average ratio of 0.326. Bioinformatic analysis showed that MIP-1α, NF-κB, TNF-α, IFN-γ and TLR4 were associated with native immunity, HWP1 associated with hyphal adhesion and formation, SAP2, SAP5, LIP4 and LIP6 associated with extracellular hydrolysis, and EFG1, CPH1 and WH11 associated with phenotypic switching. Conclusions Both the host adaptive immunity deficiency and increased virulence of Candida species are involved in the pathogenesis of vulvovaginal candidiasis, and TLR4 possibly plays a certain role in the local immunity of patients with this entity.

Key words: Pathogenesis