中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (6): 430-433.

• 论著 • 上一篇    下一篇

人皮肤鳞状细胞癌A431细胞株电压门控钾通道的研究

梁渝珩1,熊霞2,曾世华3   

  1. 1. 重庆市垫江县人民医院皮肤科
    2. 四川省泸州医学院附院皮肤科
    3. 四川省成都市第二人民医院
  • 收稿日期:2010-10-27 修回日期:2010-12-07 出版日期:2011-06-15 发布日期:2011-06-02
  • 通讯作者: 熊霞 E-mail:xiongxia789@126.com

Voltage-gated potassium channel in a human skin squamous cell carcinoma cell line A431

yuheng LIANG 2,ZENG shi-hua   

  • Received:2010-10-27 Revised:2010-12-07 Online:2011-06-15 Published:2011-06-02

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摘要：

目的 探讨电压门控钾通道在人皮肤鳞状细胞癌A431细胞和人角质形成细胞HaCaT细胞中的作用。方法 噻唑蓝方法研究加入含有不同浓度四乙胺的培养基对A431细胞和HaCaT细胞的增殖的影响；提取体外培养A431细胞、HaCaT细胞以及人正常表皮组织的蛋白，ELISA方法检测电压门控钾通道蛋白（HERG）在它们中的表达，比较它们的差异。结果 四乙胺对体外培养的A431细胞和HaCaT细胞的增殖抑制效应具有时间依赖与剂量依赖的特点，当四乙胺的浓度≥10 mmol/L且作用时间≥24 h，可以使A431细胞和HaCaT细胞的增殖受到明显的抑制。HERG钾通道蛋白在A431细胞、HaCaT细胞和人正常皮肤表皮组织均有表达，平均浓度分别为（49.7114 ± 3.55696） pg/ml、（35.7471 ± 4.14696） pg/ml、（36.8857 ± 3.47810） pg/ml，组间比较差异具有统计学意义（P < 0.05）。结论 阻断电压门控钾通道可抑制A431细胞和HaCaT细胞的增殖；电压门控钾通道可能在人皮肤鳞状细胞癌细胞上的表达更显著。

关键词: HERG

Abstract:

Objective To investigate the role of voltage-gated potassium channel in the human skin squamous cell carcinoma A431 cells and human keratinocyte HaCaT cells. Methods MTT assay was performed to detect the effect of different concentrations of tetraethylammonium （TEA） on the proliferation of cultured A431 cells and HaCaT cells. Besides, enzyme-linked immunosorbent assay （ELISA） was conducted to detect the expression of HERG channel protein in A431 cells, HaCaT cells and normal human skin tissue. Results TEA inhibited the proliferation of A431 cells and HaCaT cells in a dose- and time-dependent manner. After treated with TEA （≥10 mmol/L） for 24 or more hours, the proliferation of A431 cells and HaCaT cells was obviously suppressed. A significant difference was observed in the average concentration of HERG channel protein between A431 cells, HaCaT cells and normal human skin tissue（49.7114 ± 3.55696 pg/ml, 35.7471 ± 4.14696 pg/ml, 36.8857 ± 3.47810 pg/ml, all P < 0.05）. Conclusions The block of voltage-gated potassium channel could inhibit the proliferation of A431 cells and HaCaT cells, and the expression of voltage-gated potassium channel seems to be higher in human skin squamous cell carcinoma cells.

Key words: HERG