中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (2): 121-123.

• 论著 • 上一篇    下一篇

寻常痤疮患者外周血单核细胞TLR2的表达及其与IL-8 和TNF -α的相关性研究

王忠永1,李侃2,邱盼盼2,邱会芬2,舒春梅3,高昱4   

  1. 1. 山东省滨州医学院附属医院
    2. 山东省滨州医学院附属医院皮肤科
    3. 滨州医学院附属医院
    4. 山东滨州医学院附院
  • 收稿日期:2010-05-19 修回日期:2010-06-18 出版日期:2011-02-15 发布日期:2011-02-10
  • 通讯作者: 王忠永 E-mail:byzhongyong@sohu.com,huifenqiu@sohu.com

Expression of TLR2 in peripheral blood mononuclear cells and its correlation with interleukin-8 and tumor necrosis factor-α in patients with acne vulgaris

  • Received:2010-05-19 Revised:2010-06-18 Online:2011-02-15 Published:2011-02-10

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摘要：

目的 探讨Toll样受体2（TLR2）在寻常痤疮发病中的作用及意义。方法 选择轻、中、重度痤疮患者各30例，采用流式细胞仪检测其外周血CD14+单核细胞TLR2的表达，并采用双抗体夹心酶联免疫吸附法（ELISA）检测血清IL-8和TNF-α的水平。另取30例正常人作为对照组。结果 轻、中、重度组痤疮患者外周血单核细胞表面TLR2的表达均显著高于正常人对照组（P < 0.01）。各组患者血清中IL-8和TNF-α的水平也显著高于正常人对照组（P < 0.01）。直线相关分析显示，各组患者TLR2的表达与IL-8、TNF-α水平均呈正相关性（r分别为0.382、0.517、0.436、0.641、0.725、0.593，P < 0.05）。痤疮严重程度与TLR2的表达呈正相关性（r = 0.406，P < 0.01）。结论 TLR2表达水平及相关细胞因子浓度与痤疮严重程度密切相关。

关键词: 肿瘤坏死因子α

Abstract:

Objective To explore the role of Toll like receptor 2 （TLR2） in the pathogenesis of acne vulgaris. Methods Venous blood was collected from 30 normal human controls and 90 patients with acne vulgaris. The patients were equally divided into three groups, i.e., mild, moderate and severe groups, according to disease severity. Flow cytometry was performed to detect the expression of TLR2 in PBMCs, and double antibody sandwich ELISA to measure the levels of serum interleukin-8 （IL-8） and tumor necrosis factor-α （TNF-α）. Results A significant increase was observed in the expression of TLR2 in PBMCs, serum level of IL-8 and TNF-α in the three groups of patients with acne vulgaris compared with the normal human controls （all P < 0.01）. The expression of TLR2 was positively correlated with the expression of IL-8 （r = 0.382, 0.517, 0.436, respectively, all P < 0.05） and TNF-α （r = 0.641, 0.725, 0.593, respectively, all P < 0.05） in the patients with mild, moderate and severe acne vulgaris, respectively, and with the severity of acne vulgaris （r = 0.406, P < 0.01）. Conclusion The expression level of TLR2 and related cytokines seems closely correlated with the severity of acne vulgaris.

Key words: tumor necrosis factor-α