中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (2): 124-126.

• 论著 • 上一篇    下一篇

山东汉族梅毒患者与HLA-A、B等位基因的相关性研究

蒋红伟,田洪青,李中伟,等   

  1. 山东省皮肤病性病防治研究所
  • 收稿日期:2010-04-26 修回日期:2010-06-07 出版日期:2011-02-15 发布日期:2011-02-10
  • 通讯作者: 田洪青 E-mail:tianhq2006@126.com

Association of HLA-A and -B alleles with syphilis in Shandong Han population

  • Received:2010-04-26 Revised:2010-06-07 Online:2011-02-15 Published:2011-02-10

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摘要：

目的 探讨山东汉族梅毒患者与HLA-A、B等位基因的相关性。 方法 采用PCR-序列特异性寡核苷酸探针杂交（PCR-SSOP）方法对205例山东汉族梅毒患者与5844例山东汉族正常对照的HLA-A、B等位基因表现频率进行检测。结果 梅毒患者组HLA-A*02，B*15、40等位基因频率高于对照组（P值均 ＜ 0.01；Pc值均 ＜ 0.05），HLA-A*26等位基因频率低于对照组（P = 0.003；Pc = 0.039），HLA-B*15、40等位基因频率在显性梅毒组高于对照组（P值均 ＜ 0.01；Pc值均 ＜ 0.05），HLA-A*02、11、29，B*15、40等位基因频率在隐性梅毒组高于对照组（P值均 < 0.01；Pc值均 ＜ 0.05），HLA-A*30、33等位基因频率在隐性梅毒组低于对照组（P值、Pc值分别为0.002、0.026；0.001、0.013），HLA-A*30等位基因频率在显性梅毒组高于隐性梅毒组（P = 0.001；Pc = 0.013）。结论 HLA-A*02，B*15、40等位基因可能与山东汉族梅毒相关，HLA-A*30可能与山东汉族显性梅毒相关，HLA-A*02、11、29可能与山东汉族隐性梅毒相关。

关键词: 聚合酶链反应-序列特异性寡核苷酸探针杂交

Abstract:

Objective To investigate the association of HLA-A and -B alleles with syphilis in Shandong Han population. Methods The allele frequencies of HLA-A and -B were detected in 205 patients with syphilis and 5844 normal human controls by PCR-sequence specific oligonucleotide probe （PCR-SSOP） method. Results The patients with syphilis showed a higher frequency of HLA-A*02, B*15, B*40 alleles （all P < 0.01, Pc < 0.05） and a lower frequency of HLA-A*26 allele （P = 0.003, Pc = 0.039） than the normal human controls did. There was an increased frequency of HLA-B*15 and B*40 alleles in patients with symptomatic syphilis （both P < 0.01, Pc < 0.05）, as well as an elevated frequency of HLA-A*02, 11, 29, B*15 and 40 alleles （all P < 0.01, Pc < 0.05） and a decreased frequency of HLA-A*30 and 33 in patients with asymptomatic syphilis （P = 0.002, 0.026, Pc = 0.001, 0.013 respectively）, compared with the normal human controls. The frequency of HLA-A*30 allele was significantly higher in patients with symptomatic syphilis than in those with asymptomatic syphilis （P = 0.001, Pc = 0.013）. Conclusions There seems to be an association between HLA-A*02, B*15 and B*40 alleles and syphilis, between HLA-A*30 allele and symptomic syphilis, and between HLA-A*02, 11 and 29 alleles and asymptomatic syphilis, in Shandong Han population.

Key words: PCR-SSOP