中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (10): 720-723.

• 论著 • 上一篇    下一篇

点阵Er:YAG激光照射对皮肤胶原增生的影响

邬宗周1,袁定芬2,邓辉2,徐佩红3   

  1. 1. 上海市皮肤病医院
    2. 上海交通大学附属第六人民医院
    3.
  • 收稿日期:2010-10-06 修回日期:2011-06-03 出版日期:2011-10-15 发布日期:2011-09-30
  • 通讯作者: 袁定芬 E-mail:yuandingfen@medmail.com.cn

Effect of fractional Er:YAG laser on skin collagen proliferation

  • Received:2010-10-06 Revised:2011-06-03 Online:2011-10-15 Published:2011-09-30

PDF

35

赞

摘要：

目的 探讨点阵铒：钇铝石榴石（Er:YAG）激光定点多重脉冲照射对正常人皮肤的组织学影响。 方法 使用点阵Er:YAG激光连续多脉冲照射正常受试者的上臂皮肤，取照射后1 h、24 h、48 h、72 h、7 d、15 d及30 d的照射皮肤和未照射皮肤进行组织学观察，包括HE染色、抗热休克蛋白70、47免疫组化染色和Masson 染色。结果 点阵Er:YAG激光连续多脉冲照射正常受试者皮肤，刺激皮肤组织产生一系列点阵排列的局灶性锥状气化剥脱微损伤。照射后1 ~ 7 d，微损伤区皮肤产生炎症反应，表皮细胞上皮化；7 ~ 30 d局部真皮内胶原新生、重塑。热休克蛋白70在照射后72 h至第7天内呈峰值表达；热休克蛋白47在照射后15 ~ 30 d持续高表达。结论 点阵Er:YAG激光定点多重脉冲照射人体皮肤可以到达真皮深层，从而获得局灶性胶原增生的疗效。

关键词: 胶原

Abstract:

Objective To investigate the histological changes of normal human skin after fixed irradiation with multiple-pulse fractional Er:YAG laser. Methods After hair removal, the upper arms of 7 healthy volunteers were consecutively irradiated with multiple-pulse fractional Er:YAG laser. Tissue samples were resected from the irradiated skin at 1, 24, 48, 72 hours, on day 5, 7, 15, and 30 after the irradiation. Hematoxylin and eosin （HE） staining and Masson staining were performed to observe the histological changes and collagen proliferation respectively. Immunohistochemical staining was used to detect the expression of heat shock protein （HSP） 70 and 47. Results The consecutive irradiation with multi-pulse fractional Er:YAG laser generated an array of tapering microscopic treatment zones（MTZs） of gasification and ablation in the upper arm skin. After the irradiation, inflammation developed in the microscopic lesions with the epithelization of epidermal cells within 7 days; local dermal collagen was renewed and remodelled during the 7th to 30th day. HSP70 expression peaked as early as 48 hours after the treatment and maintained until the 7th day, while the high expression of HSP47 persisted from the 15th to 30th day after irradiation. Conclusion The fixed, multiple-pulse and fractional Er:YAG laser irradiation can reach the deep dermis, and induce the local proliferation of dermal collagen.

Key words: collagen