中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (9): 614-616.

• 论著 • 上一篇    下一篇

人乳头瘤病毒DNA在角化棘皮瘤中的表达

陈学超1,卢宪梅2,周桂芝1   

  1. 1. 济南市山东省皮肤病性病防治研究所
    2. 济南市山东省皮肤病防治研究所
  • 收稿日期:2010-01-13 修回日期:2010-04-29 发布日期:2010-09-10
  • 通讯作者: 卢宪梅 E-mail:luxianmei2003@yahoo.com.cn
  • 基金资助:

    山东省科技攻关计划项目

Expression of human papillomavirus DNA in keratoacanthoma tissue

  • Received:2010-01-13 Revised:2010-04-29 Published:2010-09-10

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摘要：

目的 探讨角化棘皮瘤组织中人乳头瘤病毒（HPV）感染率，及HPV DNA阳性细胞的分布特征。方法 原位杂交方法检测46例KA和34例正常人皮肤石蜡切片组织标本中HPV6/11、16/18、31/33亚型。结果 31例角化棘皮瘤黏膜型HPV DNA阳性，总阳性率67.39%，两种以上HPV混合感染达83.87％（26/31），各亚型均以游离型为主；阳性信号位于细胞核内或核边缘，阳性细胞多分布于火山口底部棘层的浅中部及其周围唇部，分布形式多为带状、片状或灶状聚集。正常皮肤对照组HPV DNA各亚型检测均阴性。结论 角化棘皮瘤患者HPV6/11、16/18、31/33感染率高于正常人群，且以游离形式存在为主，推测HPV感染在角化棘皮瘤的发病机制中起作用。

关键词: [关键词] 角化棘皮瘤, 乳头状瘤病毒，人, 原位杂交

Abstract:

Objective To detect the infection rate of human papillomavirus （HPV） types in keratoacanthoma （KA） tissue, and to study the distribution of HPV DNA-positive cells. Methods In situ hybridization was performed to detect HPV subtypes 6/11, 16/18, and 31/33 in paraffin-embeded tissue samples from 46 patients with KA and 34 nomal human controls. Results Mucosal HPV DNA was positive in 31 （67.39%） of the 46 KA samples, and mixed infection rate of HPV amounted to 83.87% （26/31） in these positive samples. The DNA of tested mucosal HPV types existed mainly in episomal status. Positive reactions were observed within or at the edge of nuclei. HPV infected cells showed a stripe-, slice-like or focal distribution mainly in superficial middle lamina of spinous layer at the bottom or lateral margin of crater-shaped epidermal depression. No mucosal HPV DNA was noted in normal control samples. Conclusions Compared with normal controls, patients with KA are infected with mucosal HPV at a higher frequency, and mucosal HPV DNA mainly exists in episomal status in KA tissue, hinting that HPV infection play a certain role in the pathogenesis of KA.

Key words: [Key words] Keratoacanthoma, Papillomavirus, human, In situ hybridization