中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (7): 497-500.

• 论著 • 上一篇    下一篇

卡介菌多糖核酸对二硝基氯苯诱导的Nc/Nga小鼠特应性皮炎样皮损的影响

郭亚南1,文海泉2,张桂英2,王萍3,肖嵘4   

  1. 1. 中南大学湘雅二医院
    2. 长沙中南大学湘雅二医院皮肤科
    3.
    4. 长沙中南大学湘雅二医院皮肤性病科
  • 收稿日期:2009-11-04 修回日期:2010-03-09 出版日期:2010-07-15 发布日期:2010-07-13
  • 通讯作者: 郭亚南 E-mail:pecang@163.com

Effects of BCG-PSN on 2,4-dinitrochlorobenzene-induced atopic dermatitis-like skin lesions in Nc/Nga mice

  • Received:2009-11-04 Revised:2010-03-09 Online:2010-07-15 Published:2010-07-13
  • Contact: GUO Ya-Nan E-mail:pecang@163.com

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摘要：

目的 观察卡介菌多糖核酸（BCG-PSN）对二硝基氯苯（DNCB）诱导的Nc/Nga小鼠特应性皮炎样皮损的影响。方法 15只Nc/Nga小鼠随机分成3组，每组5只。第1周开始，对照组在小鼠足垫及除毛之后的腹部外涂丙酮并腹腔注射生理氯化钠溶液100 μl，模型组外涂丙酮配置的5% DNCB并腹腔注射生理氯化钠溶液100 μl，治疗组外涂丙酮配置的5% DNCB并腹腔注射100 mg/L的BCG/PSN 100 μl，三组均为隔日给药1次，共7周。第2周开始，用丙酮配置的0.1% DNCB涂抹在小鼠双侧耳部和颈部皮肤，每周1次，共6周。通过小鼠耳朵厚度、组织病理学、免疫学指标评价皮炎损伤程度，观察BCG-PSN对小鼠的治疗作用。结果 外用DNCB可以引起Nc/Nga小鼠湿疹样皮炎的产生，模型组小鼠出现皮肤干燥、红斑、水肿、脱毛和糜烂，组织病理学显示表皮和真皮增厚、过度角化和炎症细胞浸润，小鼠血清IgE（174.72 ± 12.64 μg/L）和IL-4（91.49 ± 6.32 ng/L）增高，与对照组IgE（17.32 ± 3.56 μg/L）和IL-4（83.95 ± 6.63 ng/L）相比，P值均 ＜ 0.05；而治疗组小鼠血浆IL-12（122.10 ± 4.64 ng/L）和IFN-γ（73.89 ± 2.39 ng/L）增加，IgE（84.27 ± 9.35 μg/L）下降，与模型组IL-12（20.14 ± 6.15 ng/L）、IFN-γ（51.53 ± 3.45 ng/L）和IgE相比，差异均有统计学意义。结论 BCG-PSN可能通过促进IL-12和 IFN-γ分泌，抑制IgE合成，发挥治疗Nc/Nga小鼠特应性皮炎样皮损的作用。

关键词: Nc/Nga鼠, 特应性皮炎, 模型, 卡介菌多糖核酸

Abstract:

Objective To determine the effect of bacille Calmette-Guerin-polysaccharide nucleic acid （BCG/PSN） on 2,4-dinitrochlorobenzene （DNCB）-induced atopic dermatitis-like skin lesions in Nc/Nga mice. Methods Fifteen mice were randomly and equally classified into 3 groups, i.e., control group receiving topical acetone on foot pad and abdomen and intraperitoneal injection of physiological saline, model group receiving topical 5% DNCB solution and intraperitoneal injection of physiological saline, treatment group receiving 5% DNCB solution and intraperitoneal injection of BCG/PSN, and all drugs were used every other day for 7 weeks. Further more, 0.1% DNCB was topically applied on the ear and neck of Nc/Nga mice once a week from week 2 to week 7. The effects of BCG/PSN were evaluated by ear thickness, skin histopathology and immunological parameters. Results Repeated application of DNCB caused the development of eczematous dermatitis in mice. Mice in model group clinically manifested skin dryness, erythema, edema and erosion with histopathological changes including dermal and epidermal thickening, hyperkeratosis, and inflammatory infiltration. The serum levels of IL-4 and IgE in model group were significantly higher than those in control group［（174.72 ± 12.64） μg/L vs （17.32 ± 3.56） μg/L, （91.49 ± 6.32） ng/L vs （83.95 ± 6.63） ng/L, both P ＜ 0.05］. Increased serum IL-12 and IFN-γ and decreased serum IgE were observed in treatment group compared with the model group［（122.10 ± 4.64） ng/L vs （20.14 ± 6.15） ng/L, （73.89 ± 2.39） ng/L vs （51.53 ± 3.45） ng/L, （84.27 ± 9.35） μg/L vs （174.72 ± 12.64） μg/L, all P ＜ 0.05］. Conclusion BCG/PSN might be beneficial for the treatment of atopic dermatitis-like skin lesions in Nc/Nga mice by enhancing the secretion of IL-12 and IFN-γ and suppressing the synthesis of IgE.

Key words: Nc/Nga mice, atopic dermatitis, model, BCG-PSN