中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (7): 471-473.

• 论著 • 上一篇    下一篇

转录因子Aiolos基因在SLE患者外周血单一核细胞中的表达

刘样满1,陈晓红1,杨莹2,曹碧兰1,袁伟3,文国容4,曾令芬4   

  1. 1. 遵义医学院附属医院皮肤科
    2. 贵州省遵义医学院附属医院皮肤科
    3. 遵义医学院附属医院
    4.
  • 收稿日期:2009-11-13 修回日期:2009-12-14 出版日期:2010-07-15 发布日期:2010-07-13
  • 通讯作者: 刘样满 E-mail:821750628@qq.com
  • 基金资助:

    贵州省科技厅资助项目

Expression of Aiolos transcription factor in peripheral blood mononuclear cells （PBMCs） from patients with systemic lupus erythematosus （SLE）

  • Received:2009-11-13 Revised:2009-12-14 Online:2010-07-15 Published:2010-07-13
  • Contact: Liu yangman E-mail:821750628@qq.com

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摘要：

目的 探讨SLE患者外周血单一核细胞（PBMC）中转录因子Aiolos的表达水平。方法 采用Western印迹法分别检测19例SLE活动期患者及13例SLE稳定期患者PBMC中转录因子Aiolos蛋白的表达水平，并以30例健康志愿者作对照。对SLE患者Aiolos蛋白表达水平与SLE疾病活动指数（SLEDAI）进行相关分析。结果 PBMC中转录因子Aiolos蛋白表达相对值正常对照组为0.81 ± 0.09，SLE组为0.56 ± 0.17，两组比较差异有统计学意义（P < 0.01）；SLE活动期组（0.52 ± 0.14）与稳定期组（0.65 ± 0.19）比较，差异有统计学意义（P < 0.05）；SLE患者转录因子Aiolos蛋白的表达与SLEDAI呈显著负相关（r = -0.65，P < 0.01）。结论 SLE患者PBMC中转录因子Aiolos的表达水平显著降低，且与SLE疾病活动指数呈一定相关性。

关键词: 红斑狼疮,系统性, 转录因子Aiolos

Abstract:

Objective To investigate the expression of Aiolos transcription factor in peripheral blood mononuclear cells （PBMCs） from patients with systemic lupus erythematosus （SLE）, and to explore their clinical significance. Methods Western blot was performed to detect the protein expression of Aiolos transcription factor in PBMCs from 19 patients with active SLE, 13 patients with stable SLE and 30 healthy volunteers. The relationship between the expression of Aiolos transcription factor and SLE disease activity index （SLEDAI） was analyzed. Results A significant difference was noted in the relative expression of Aiolos transcription factor between SLE patients and normal controls （0.56 ± 0.17 vs 0.81 ± 0.09, P < 0.01） and between patients in active stage and those in stable stage （0.52 ± 0.14 vs 0.65 ± 0.19, P < 0.05）. In addition, the expression level of Aiolos protein was negatively correlated with SLEDAI in patients（r = -0.65, P < 0.01）. Conclusion A decrease is observed in the expression of Aiolos transcription factor in SLE patients, which is correlated with the disease activity in SLE.

Key words: Lupus erythematosus,systemic, transcription factor Aiolos