中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (5): 363-365.

• 短篇论著 • 上一篇    下一篇

质粒介导产β－内酰胺酶淋球菌的检测及其TEM-1基因质粒分型

吴兴中1,郑和平2,黄进梅3,曾维英3,薛耀华4,潘慧清5   

  1. 1. 广东省皮肤性病防治中心
    2. 广州市广东省皮肤性病防治中心
    3. 广州市广东省皮肤病防治中心
    4. 广州市广东省皮肤病防治研究所
    5. 广州广东省皮肤病防治研究所
  • 收稿日期:2009-07-10 修回日期:2009-08-12 出版日期:2010-05-15 发布日期:2012-04-12
  • 通讯作者: 吴兴中 E-mail:wwxingzhong@yahoo.com.cn

Detection of and typing of plasmids carrying TEM-1 gene in plasmid-mediated beta-lactamase-producing Neisseria gonorrhoeae strains

  • Received:2009-07-10 Revised:2009-08-12 Online:2010-05-15 Published:2012-04-12

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摘要：

【摘要】 目的 调查质粒介导的产? －内酰胺酶淋球菌 (PPNG)在淋球菌临床分离株中流行情况及TEM-1基因的分子流行病学情况。方法 收集2000-2008年从性病门诊患者中分离的 934株淋球菌，纸片酸度法测定PPNG，多重PCR方法鉴定?－内酰胺酶质粒并行TEM-1基因分型。 结果 2000-2008年共检测934株淋球菌，检出230株PPNG(24.6%)，流行率逐年增多，由2000年的17.1%上升到2008年的42.1%；PPNG的TEM-1基因质粒分型以亚洲型质粒流行为主，只在2008年检测出1株含非洲型质粒，未见多伦多型质粒。结论 PPNG流行率逐年增高，产? －内酰胺酶的TEM-1基因流行以亚洲型质粒为主，偶见非洲型质粒。

关键词: TEM-1

Abstract:

【ABSTRACT】 Objective To investigate the prevalence and molecular epidemiology of penicillinase (β-lactamase) -producing Neisseria gonorrhoeae (PPNG) in Guangzhou. Methods： A total of 934 clinical isolates of Neisseria gonorrhoeae were consecutively collected from outpatients with gonorrhoea attending STD clinics from 2000 to 2008. PPNG were detected by paper acidometric method. A multi-PCR was used to distinguish subtypes of TEM- 1 gene of β–lactamase plasmids of PPNG. Results: Out of 934 Neisseria gonorrhoeae isolates examined, 230 (24.6%) strains were PPNG. The prevalence of PPNG increased year by year, from 17.1% to 42.1% (χ2=10.8, P>0.01) in 2000-8. Subtyping TEM-1 gene of β-lactamase plasmids of PPNG showed that 229 (99.6%) PPNGs carried the Asian-type plasmids but only one African-type plasmid was found in 2008. No isolates with Toronto/Rio-type plasmids were detected. Conclusion: The prevalence of PPNG increased year by year. The Asian-type β–lactamase plasmids in PPNG were dominated, while African-type plasmid was sporadic in Guangzhou.

Key words: TEM- 1