中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (5): 362-363.

• 短篇论著 • 上一篇    下一篇

生殖器疱疹患者外周血单核细胞Toll样受体的表达

付金玲1,李其林2,黎庆梅3,尚智伟2,李晓辉2,李湘君3   

  1. 1. 暨南大学第四附属医院/广州市红十字会医院
    2. 暨南大学第四附属医院，广州市红十字会医院皮肤科
    3.
  • 收稿日期:2009-07-17 修回日期:2009-08-16 出版日期:2010-05-15 发布日期:2012-04-12
  • 通讯作者: 付金玲 E-mail:fjl378@yahoo.com.cn

Expression of Toll-like receptors on peripheral monocytes from patients with genital herpes

  • Received:2009-07-17 Revised:2009-08-16 Online:2010-05-15 Published:2012-04-12

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摘要：

【摘要】 目的 探讨生殖器疱疹反复发作的免疫学机制。方法 将42例RGH患者分为年复发次数≧6次和年复发次数<6次两个亚组，采用双色免疫荧光抗体染色流式细胞术检测其外周血单核细胞TLR4、TLR8、TLR9的表达及T淋巴细胞亚群，采用双抗体夹心ELISA法检测其外周血IFN-?、IL-2、IL-12水平，并均以30例正常人作为对照。结果 RGH组外周血单核细胞TLR4表达增高，差异具有统计学意义（?<0.05）, TLR8表达降低，差异具有统计学意义（?<0.05）；RGH患者外周血IFN-?、IL-2、IL-12水平以及CD3+ T细胞、CD4+ T细胞、CD4+/CD8+比值均下降，差异具有统计学意义（?<0.05）。结论 TLR4可能为HSV的识别受体,在机体抗HSV感染中发挥重要的识别作用，检测外周血单核细胞TLR4表达的变化有可能成为HSV感染观测指标之一；TLR8的表达下降,机体产生Th1型细胞因子障碍,可能为GH反复发作的免疫学机制之一；机体细胞免疫功能低下可能是GH反复发作的重要原因。

关键词: 单核细胞

Abstract:

【Abstract】 Objective Discussing the immunological mechanisms of repeated recurrence in genital herpes. Methods Dividing 42 cases of patients with RGH into two subgroups with recurrence number ≧6 times and recurrence number <6 times, Detecting the expression of TLR4、TLR8、TLR9 and T lymphocyte subsets in peripheral blood using two color immuno-fluorescence antibody staining flow cytometry,Detecting the level of IFN-?、IL-2、IL-12 in peripheral blood by Double antibody sandwich ELISA assay,and 30 cases are normal as a control.Results The expression of TLR4 on peripheral blood mononuclear cells of RGH group is higher than the control group,there is a significant difference（?<0.05）, The expression of TLR8 in RGH group is lower than the control group , there is a significant difference（?<0.05）; The level of IFN-?、IL-2、IL-12 and CD3+T cells、CD4+T cells、the ratio of CD4+/CD8+in peripheral blood of patients with RGH are all decreasing, there is a significant difference（?<0.05）. Conclusion TLR4 is the the possible receptor of recognition for HSV, It plays an important role in recognizing HSV,Detecting the expression of TLR4 on peripheral blood mononuclear cells may be one of observation indexs for HSV infection;The expression of TLR8 is decreasing , suggest the body may not produce effectivly Th1 type cytokines when a large number replication of HSV, and it may be one of the immunological mechanisms for recurrence of GH; cellular immune dysfuction is an important factor for recurrence of GH.