中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (2): 82-84.

• 论著 • 上一篇    下一篇

microRNA在人皮肤黑素瘤与色素痣中共同差异表达的研究

王焱1,孙建方1,方方1,韩峻松2,张国成1   

  1. 1. 南京 中国医学科学院北京协和医学院皮肤病研究所
    2. 生物芯片上海国家工程研究中心
  • 收稿日期:2009-06-12 修回日期:2009-08-05 出版日期:2010-02-15 发布日期:2012-03-30
  • 通讯作者: 王焱 E-mail:wang1890@yahoo.com.cn
  • 基金资助:
    财政部中央级公益性科研院所基本科研业务专项基金;江苏省“六大高峰”人才基金

Identification of microRNAs differentially expressed in human cutaneous malignant melanoma and pigmented nevi by microRNA microarray

  • Received:2009-06-12 Revised:2009-08-05 Online:2010-02-15 Published:2012-03-30

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摘要： 目的 筛选人皮肤黑素瘤组织中表达的已知hsa-microRNA。方法 采用SBC microRNA芯片技术，将6例人皮肤黑素瘤组织总RNA分别与9例健康人色素痣总RNA混合物对比，筛选已知的468条hsa-microRNA表达情况。用qPCR分别检测这6例人皮肤黑素瘤组织中各候选hsa-microRNA的表达。将芯片和qPCR两种方法检测结果一致的候选hsa-microRNA确定为有意义的共同差异表达hsa-microRNA。结果 2倍以上差异表达miRNA占所检测miRNA的12.18% ～ 86.33%，5倍以上差异表达miRNA占1.28% ～ 19.02%，10倍以上差异表达miRNA占0.43% ～ 5.34%。 此6例人皮肤黑素瘤中miRNA-21 明显上调表达，miRNA-320和miRNA-494明显下调表达。结论 人皮肤黑素瘤组织中miRNA-21明显上调表达，miRNA-320和miRNA-494明显下调表达。

关键词: 黑色素瘤, 微RNAs, 微芯片

Abstract: Objective To select microRNAs （miRNA） associated with human cutaneous malignant melanoma （MM）. Methods Total RNA was extracted from 6 tissue samples of MM and 9 human control samples of pigmented nevi, and small RNAs of less than 200 bp were enriched. miRNA microarray was used to select differentially expressed miRNAs between tissue samples of MM and pigmented nevi from 468 candidates. The expression of differentially expressed miRNAs was confirmed by fluorescence based real-time quantitative PCR （qPCR） in all of these samples. Those miRNAs that were identified as differentially expressed with both miRNA microarray and qPCR were considered as significant miRNAs. Results Between the tissue samples of MM and pigmented nevi, 12.18% to 86.33% of miRNAs differentially expressed by more than 2 folds, 1.28% to 19.02% by more than 5 folds, and 0.43% to 5.34% by more than 10 folds. The expression of miRNA-21 was obviously up-regulated, while that of miRNA-320 and miRNA-494 was down-regulated in the MM samples. Conclusion There is an increase in the expression of homo sapiens miRNA-21 but a decrease in that of miRNA-320 and miRNA-494 in MM tissues.

Key words: Melanoma, MicroRNAs