中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (12): 871-872.

• 短篇论著 • 上一篇    下一篇

慢性特发性荨麻疹患者外周血调节性T细胞和IL-17水平的研究

沈斌1,冷建杭2,王克义2,吴黎明3,卜璋于4,李雅芬5,陈健杭州5,苏明5   

  1. 1. 浙江省杭州市第一人民医院
    2.
    3. 杭州市第一医院皮肤科
    4. 杭州浙江大学医学院附属第二医院皮肤科
    5. 杭州市第一人民医院皮肤科
  • 收稿日期:2010-04-08 修回日期:2010-06-02 出版日期:2010-12-15 发布日期:2010-12-13
  • 通讯作者: 沈斌 E-mail:shenbin@hz.cn

Detection of peripheral blood regulatory T cells and serum interleukin-17 in patients with chronic idiopathic urticaria

  • Received:2010-04-08 Revised:2010-06-02 Online:2010-12-15 Published:2010-12-13

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摘要：

[摘要] 目的 探讨慢性特发性荨麻疹患者外周血中CD4+CD25+调节性T细胞数量和IL-17水平及其与病情的关系。方法 慢性特发性荨麻疹患者和正常对照各30例，用流式细胞仪检测外周血中CD4+CD25+调节性T细胞数量，ELISA法检测血清中IL-17的水平。结果 慢性特发性荨麻疹患者外周血调节性T细胞的数量明显低于正常人对照组，差异有统计学意义（t=3.021，P﹤0.01）；慢性特发性荨麻疹患者血清中IL-17水平(8.1±0.9)pg／mL高于健康对照组血清中的IL—17水平(6.7±1.1)pg／mL，差异有统计学意义（t =3.365, P﹤0.01）；慢性特发性荨麻疹患者外周血调节性T细胞的数量和病情评分无相关性(r=-0.1012 , P >0.05)，而血清中IL-17水平和病情评分呈正相关（r=0．6586, P <0.05)。结论 慢性特发性荨麻疹患者外周血调节性T细胞的数量下降和血清中IL-17水平升高。推测慢性特发性荨麻疹患者体内调节性T细胞和Th17细胞二个亚群存在失衡，在慢性特发性荨麻疹发病中起着重要的作用。

关键词: 荨麻疹, T淋巴细胞, 白细胞介素17

Abstract:

[Abstract] Objective To explore the populations of circulating CD4+CD25+regulatory T cells and serum levels of IL-17 in patients with chronic idiopathic urticaria. Methods The populations of circulating CD4+CD25+regulatory T cells were detected with flow cytometric assay and the serum levels of IL-17 were measured by ELISA in 30 patients with chronic idiopathic urticaria and 30 normal controls. Results A significant decreased frequency of CD4+CD25+regulatory T cells was observed in patients with chronic idiopathic urticaria compared to normal controls（t =3.021，P<0.01）;While the serum levels of IL-17 were higher in patients with chronic idiopathic urticaria than that in normal controls（t =3.365，P<0.01）.Furthermore ,the serum levels of IL-17 were positively related to the disease score (r=0.6586, P <0.05). Conclusion Our data demonstrate that there is an imbalance between CD4+CD25+regulatory T cells and Th17 cells in the patients with chronic idiopathic urticaria, and that may play an important role in the pathogenesis of chronic idiopathic urticaria. Factors regulating apoptosis and homeostasis of CD4+CD25+regulatory T cells and Th17 cells are new therapeutic targets.