中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (9): 625-627.

• 论著 • 上一篇    下一篇

人IFNα-2b对HPV11-E7 DNA疫苗的免疫佐剂效应

相文忠1,王飞2,康健3,等4   

  1. 1. 杭州市第三人民医院皮肤科
    2. 南京东南大学附属中大医院皮肤科
    3. 南京医科大学第一附属医院（江苏省人民医院）
    4. 湖北省中山医院整形美容外科
  • 收稿日期:2008-08-27 修回日期:2009-02-02 出版日期:2009-09-15 发布日期:2012-03-21
  • 通讯作者: 相文忠 E-mail:xiangwenzhong@126.com
  • 基金资助:
    江苏省重点学科基金资助项目(135-03)；国家自然科学基金资助项目(30100156)

Role of human interferon α-2b as an immunological adjuvant for human papillomavirus （HPV） 11-E7 DNA vaccine

Xiang WenZhongfei wang2,Kang Jian 3   

  • Received:2008-08-27 Revised:2009-02-02 Online:2009-09-15 Published:2012-03-21
  • Contact: Xiang WenZhong E-mail:xiangwenzhong@126.com

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摘要： 目的 探讨人IFNα-2b对HPV11-E7 DNA疫苗的免疫佐剂效应。方法 将28只BALB/c小鼠随机分为4组，每组7只。融合基因组pcDNA3.1（+）-IFNα-2b-HPV11-E7组，分别肌内注射。初次免疫后2周以同样方法和相同剂量加强免疫，2次免疫后再以同样方法和相同剂量加强免疫。于6周后剖杀BALB/c小鼠，取脾并分离脾淋巴细胞，进行细胞培养并刺激单个核细胞产生细胞因子，用流式细胞仪检测Th1、Th2细胞因子比例。结果 融合基因组pcDNA3.1（+）-IFNα-2b-HPV11-E7组的IFN-γ水平明显高于pcDNA3.1（+）-HPV11-E7组，差异具有统计学意义（P < 0.001）；融合基因组与pcDNA3.1（+）-HPV11-E7组的IFN-γ水平明显高于对照组PBS组及空载质粒pcDNA3.1（+）组，差异有统计学意义（P < 0.001）；两对照组之间的IFN-γ水平差异无统计学意义（P > 0.05）。各组之间的IL-4及IL-10水平差异无统计学意义（P > 0.05）。结论 人IFNα-2b能明显加强HPV11-E7的免疫原性，提高免疫小鼠的Ｔ淋巴细胞免疫能力，IFNα-2b以增强Th1型应答为主，对Th2型应答无明显影响。

关键词: 尖锐湿疣, 人乳头瘤病毒, 乳头瘤病毒E7蛋白质类, 干扰素α-2b

Abstract: Objective To investigate the role of human interferon （IFN）α-2b in cellular and humoral immune response in BALB/c mice. Methods 　　Plasmids pcDNA3.1（+）-HPV11-E7 and pcDNA3.1（+）- IFNα-2b-linker-HPV11-E7 were constructed. Twenty-eight BALB/c mice were randomly and equally divided into four groups to be immunized with pcDNA3.1（+）-IFNα-2b-linker-HPV11-E7 （group D）, pcDNA3.1（+）-HPV11-E7（group C）, pcDNA3.1（+）（group B）, phosphate buffered solution （group A）, respectively, by intramuscular injection. Repetitive immunization was carried out on week 2 and 4. On week 6, mice were killed, splenocytes were isolated, cultured, and stimulated followed by the detection of expression level of IFN-γ, interleukin （IL）-4 and -10 in CD3+ cells and CD4+ cells. Results The level of IFN-γ （expressed as the absorbance at 460 nm） in group C was significantly higher than that in groups A and B （4.36 ± 0.42 vs 3.20 ± 0.41 and 3.29 ± 0.35, both P < 0.001）, but lower than that in group D （4.36 ± 0.42 vs 6.39 ± 0.05, P < 0.001）, and there was no significant difference between group A and B （P > 0.05）. No significant difference was noted in the levels of IL-4 or IL-10 among these 4 groups （P > 0.05）. Conclusions IFNα-2b can enhance the immunogenicity of HPV11-E7 and strengthen cellular immunity, especially Th1-type immune response to HPV11-E7 DNA vaccine in mice.

Key words: Condyloma Acuminatum, Human papilloma virus