中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (9): 613-615.

• 论著 • 上一篇    下一篇

SLE患者干扰素调节因子5和Toll样受体9 mRNA的表达

宋卫青1,李化会1,陈华波1,等2   

  1. 1. 山东省青岛市市立医院
    2. 湖北省中山医院整形美容外科
  • 收稿日期:2008-11-18 修回日期:2008-12-22 出版日期:2009-09-15 发布日期:2012-03-21
  • 通讯作者: 宋卫青 E-mail:lhh39153@163.com
  • 基金资助:
    青岛市科技局，合同编号05-1-NS-77;不符合上述请自行填写具体基金资助项目名称（编号）

mRNA expression of interferon regulatory factor 5 and Toll like receptor-9 in patients with systemic lupus erythematosus

  • Received:2008-11-18 Revised:2008-12-22 Online:2009-09-15 Published:2012-03-21

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摘要： 目的 探讨SLE患者和正常人干扰素调节因子5（IRF5）、Toll样受体9（TLR-9）基因表达的差异。方法 提取92例活动期的SLE患者与88例正常人对照外周血总RNA并反转录为cDNA，采用实时荧光定量PCR扩增IRF5、TLR-9基因，获得扩增体系的Ct值后，用公式2-?驻?驻Ct计算SLE组和正常人对照组的IRF5、TLR-9的mRNA表达水平。SLE患者抗ds-DNA抗体检测采用免疫印迹法。结果 实时荧光定量PCR扩增得到预期大小的IRF5、TLR-9基因片段。SLE组外周血IRF5、TLR-9 mRNA的表达分别是0.232 ± 0.076、0.189 ± 0.054，正常人对照组两基因mRNA的表达分别是0.081 ± 0.040、0.066 ± 0.023；两组比较，SLE组IRF5、TLR-9 mRNA表达水平升高（P均 < 0.05）。SLE患者抗ds-DNA抗体阳性组IRF5、TLR-9基因表达水平有增高的趋势，但与阴性组相比差异无统计学意义。结论 SLE患者外周血IRF5、TLR-9表达异常，可能在发病中起一定作用。

关键词: 红斑狼疮，系统性, 干扰素调节因子类, Toll样受体

Abstract: Objective To investigate the expression of interferon regulatory factor 5 （IRF5） and Toll like receptor-9 （TLR-9） in patients with systemic lupus erythematosus （SLE）. Methods General data on 92 patients with SLE and 88 normal human controls were collected. Peripheral blood was drawn from all the subjects, total RNA was extracted from the blood and transcripted to cDNA．SYBR green I real-time quantitative polymerase chain reaction （PCR） was used to amplify IRF5 and TLR-9 genes. Cycle threshold （CT） value was obtained from real-time quantitative PCR, and the mRNA expression level was expressed as the value of 2-?驻?驻CT. Anti-dsDNA antibody was detected by immunoblotting method. Results Expected fragments of IRF5 and TLR-9 genes were amplified by real-time quantitative PCR. The mRNA expression levels of IRF5 and TLR-9 were 0.232 ± 0.076 and 0.189 ± 0.054, respectively, in patients with SLE, significantly higher than those in normal controls （0.081 ± 0.040 and 0.066 ± 0.023, respectively, both P < 0.05）. The patients positive for anti-dsDNA antibody tended to have a high level of IRF5 and TLR-9 mRNA compared with those negative for anti-dsDNA antibody, but there was no statistical difference. Conclusion There is an abnormal expressions of IRF5 and TLR-9 mRNA, which may play a role in the pathogenesis of SLE.

Key words: systemic lupus erythematosus