中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (8): 537-540.

• 论著 • 上一篇    下一篇

电子传递链对白念珠菌菌丝形成的影响

刘泽虎1,吕雪莲2,李筱芳2,吕桂霞2,沈永年2,李岷2,刘维达2   

  1. 1. 中国医学科学院、中国协和医科大学皮肤病研究所
    2. 南京 中国医学科学院北京协和医学院皮肤病研究所
  • 收稿日期:2009-01-16 修回日期:2009-02-11 出版日期:2009-08-15 发布日期:2009-08-10
  • 通讯作者: 刘泽虎

Effect of electron transfer system on the hyphal formation of Candida albicans

  • Received:2009-01-16 Revised:2009-02-11 Online:2009-08-15 Published:2009-08-10

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摘要：

目的 探讨电子传递链对白念珠菌菌丝形成的影响。方法 用含10％小牛血清的RPMI 1640在5% CO2 37 ℃培养条件下诱导白念珠菌菌丝形成，观察加用电子传递链的抑制剂和激动剂对白念珠菌菌丝形成的影响、生长曲线和菌丝形成率，MTT法检测对白念珠菌的抑制率。结果 氯仿和二甲基亚砜的溶媒对照和空白对照对白念珠菌的生长和菌丝形成的影响无差异。噻吩甲酰三氟丙酮（TTFA）和苯甲羟肟酸作用于白念珠菌24 h后细胞形态以酵母为主。鱼藤酮、抗霉素A、寡霉素、叠氮钠、TTFA和丙二酸钠盐与对照组比较，在白念珠菌生长的对数期显著抑制白念珠菌的生长（P < 0.01）。苯甲羟肟酸作用于白念珠菌后显著抑制白念珠菌的生长，与空白组比较以生长对数期的抑制差异有统计学意义（P < 0.01）。鱼藤酮、抗霉素A、寡霉素、叠氮钠、TTFA、丙二酸钠盐、苯甲羟肟酸和鸟苷酸钠作用于白念珠菌12 h的菌丝形成率分别为87.49 ± 0.52、48.75 ± 4.44、50.33 ± 8.50、99.00 ± 1.00、1.60 ± 0.53、94.01 ± 0.99、0.00 ± 0.00和92.33 ± 2.08。MTT法检测鱼藤酮、抗霉素A、寡霉素、叠氮钠、TTFA、丙二酸钠盐、苯甲羟肟酸和鸟苷酸钠对白念珠菌的抑制率分别为1.34% ± 0.15%、70.61% ± 1.02%、50.63% ± 5.38%、17.80% ± 7.89%、45.17% ± 1.27%、10.75% ± 3.62%、72.46% ± 1.14%和-（5.96% ± 4.07%）。结论 经典呼吸链和替代途径电子传递链的抑制剂能不同程度抑制白念珠菌的菌丝形成，替代氧化途径是白念珠菌菌丝形成的主要途径。

关键词: 白念珠菌;菌丝;替代氧化途径

Abstract:

Objective To investigate the effect of electron transfer system on the hyphal formation of Candida albicans. Methods Candida albicans was cultured in RPMI 1640 supplemented with 10% newborn calf serum in 5% CO2 at 37 ℃ with or without the presence of inhibitors or activators of electron transfer system. Growth curve, morphology and percent of filamentation were observed for Candida albicans. MTT assay was used to assess the viability of Candida albicans. Results The solvents （chloroform and dimethyl sulfoxide） had no significant effect on the growth of and filamentation in Candida albicans. After incubation with thenoyltrifluoroacetone （TTFA） or benzhydroxamic acid for 24 hours, yeast cells of Candida albicans predominated in the culture. The growth of Candida albicans was significantly inhibited in log phase by the incubation with classic respiratory chain inhibitors such as rotenone, antimycin A, oligomycin, sodium azide, TTFA and sodium malonate, compared with the controls （all P < 0.01）. Benzhydroxamic acid, an inhibitor of alternative oxidative pathway, also significantly inhibited the growth of Candida albicans in log phase （t = 10.92, P < 0.01）. After incubation with rotenone, antimycin A, oligomycin, sodium azide, TTFA, sodium malonate, benzhydroxamic acid and disodium guanylate, the percentage of filamentation in Candida albicans at 12 hours was 87.49 ± 0.52, 48.75 ± 4.44, 50.33 ± 8.50, 99.00 ± 1.00, 1.60 ± 0.53, 94.01 ± 0.99, 0.00 ± 0.00 and 92.33 ± 2.08, respectively, and the growth of Candida albicans at 7 hours was inhibited by （1.34 ± 0.15）%, （70.61 ± 1.02）%, （50.63 ± 5.38）%, （17.80 ± 7.89）%, （45.17 ± 1.27）%, （10.75 ± 3.62）%, （72.46 ± 1.14）% and （5.96 ± 4.07）%, respectively. Conclusions Hyphal formation of Candida albicans could be suppressed by inhibitors of classic respiratory chain or alternative oxidative pathway, and is mainly regulated by alternative oxidative pathway.