中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (7): 477-480.

• 论著 • 上一篇    下一篇

α-促黑素对银屑病患者外周血单一核细胞产生TNF-α和IL-10的影响

张春华1,毕新岭2,顾军3,张莉4,等5   

  1. 1. 上海市宝山中心医院皮肤科
    2. 上海第二军医大学长海医院皮肤科
    3. 上海第二军医大学附属长海医院皮肤科
    4. 青海大学附属医院皮肤科
    5. 湖北省中山医院整形美容外科
  • 收稿日期:2008-05-19 修回日期:2009-03-02 出版日期:2009-07-15 发布日期:2009-07-08
  • 通讯作者: 张春华

Effects of α-melanocyte-stimulating hormone on the production of TNF-α and IL-10 by peripheral blood mononuclear cells from patients with psoriasis

  • Received:2008-05-19 Revised:2009-03-02 Online:2009-07-15 Published:2009-07-08
  • Contact: ZHANG Chun-Hua

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摘要：

目的　探讨α-促黑素（α-MSH）对银屑病患者外周血单一核细胞（PBMC）产生TNF-α、IL-10的影响。方法　标本取自20例寻常性银屑病患者和10例健康献血者，用不同浓度α-MSH处理经过植物血凝素（PHA）刺激的PBMC，用ELISA法检测PBMC培养上清中TNF-α、IL-10水平，应用荧光实时定量PCR分析PBMC中TNF-α mRNA、IL-10 mRNA的表达。结果　银屑病患者PBMC培养上清中TNF-α水平高于正常对照（P < 0.01），IL-10水平低于正常对照（P < 0.01）；各浓度α-MSH均可抑制TNF-α的分泌（P < 0.01），浓度为10-13 mol/L时作用最强（P < 0.01）；各浓度α-MSH均可诱导IL-10的分泌（P < 0.01，P < 0.05），浓度为10-13 mol/L时作用最强（P < 0.01）。浓度为10-13 mol/L的α-MSH下调TNF-α mRNA的表达（P < 0.01）、上调IL-10 mRNA的表达（P < 0.01）。结论　α-MSH可调节银屑病患者TNF-α和IL-10产生。

关键词: α-黑素细胞刺激素;银屑病，寻常型;外周血单个核细胞;肿瘤坏死因子-α;白介素-10

Abstract:

Objective To investigate the effects of alpha-melanocyte-stimulating hormone （alpha- MSH） on the production of tumor necrosis factor-alpha （TNF-alpha） and interleukin-10 （IL-10） by peri- pheral blood mononuclear cells （PBMCs） from patients with psoriasis vulgaris. Methods Heparinized peri- pheral blood was obtained from 20 patients with psoriasis vulgaris and 10 healthy human controls. PBMCs were isolated, cultured in complete medium, and stimulated with phytohemagglutinin （PHA） alone, the combination of PHA and various concentrations of alpha-MSH, or nothing. After another 48-hour culture, ELISA and real-time PCR were performed to measure the secretion levels of TNF-alpha and IL-10 in the supernatants of cultured PBMCs as well as the mRNA expression levels of TNF-alpha and IL-10 in PBMCs. Results The secretion level of TNF-alpha in the supernatants of patient-derived PBMCs stimulated by nothing or PHA alone was significantly higher than that from normal control-derived PBMCs （329.87 ± 99.33 ng/L vs 116.95 ± 37.15 ng/L, 1756.01 ± 183.60 ng/L vs 1287.30 ± 152.36 ng/L, both P<0.01）. alpha-MSH of all tested concentrations （10-13, 10-11, 10-9, 10-7 mol/L） could inhibit the secretion of TNF-alpha by PBMCs compared with PHA alone （all P < 0.01）, and the maximum effective concentration was 10-13 mol/L. On the contrary, a significant decrease was observed in the secretion level of IL-10 in the supernatants of patient- derived PBMCs stimulated by nothing or PHA alone compared with normal control-derived PBMCs （P < 0.05 or 0.01）. Moreover, the secretion of IL-10 by PBMCs was promoted by alpha-MSH of all tested concentrations （P < 0.01 or 0.05）, with the maximum effective concentration being 10-13 mol/L （P < 0.01）. The alpha-MSH of 10-13 mol/L down-regulated the mRNA expression of TNF-alpha （P < 0.001）, but up-regulated that of IL-10 （P < 0.001） in PHA-stimulated PBMCs from patients. Conclusion alpha-MSH can regulate the production of TNF-alpha and IL-10 by PHA-stimulated PBMCs from patients with psoriasis vulgaris.