中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (7): 457-459.

• 论著 • 上一篇    下一篇

SLE患者外周血T、B淋巴细胞TLR9蛋白表达及与临床指标的关系

吴欧1,陈国平2,陈红3,等4   

  1. 1. 安徽医科大学流行病学教研室（杭州市江干区笕桥镇明石路杭州市公共卫生中心 邮编：310006
    2. 安徽医科大学公共卫生学院分子流行病学系
    3. 合肥 安徽医科大学流行病与卫生统计学系
    4. 湖北省中山医院整形美容外科
  • 收稿日期:2008-06-10 修回日期:2009-03-07 出版日期:2009-07-15 发布日期:2009-07-08
  • 通讯作者: 吴欧
  • 基金资助:

    国家自然科学基金资助项目（编号）

Expressions of Toll-like receptor 9 protein in peripheral B and T lymphocytes from patients with systemic lupus erythematosus and their relationship with clinical parameters

  1. 1.
    2. 湖北省中山医院整形美容科
  • Received:2008-06-10 Revised:2009-03-07 Online:2009-07-15 Published:2009-07-08

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摘要：

目的 探讨SLE患者外周血B、T淋巴细胞中Toll样受体9（TLR9）蛋白的表达水平及其与相关临床指标间的关系。方法 B、T淋巴细胞及TLR9蛋白各自用荧光抗体标记，用流式细胞仪测定35例新诊断但尚未经治疗的SLE患者及16例正常人对照外周血B、T淋巴细胞中TLR9蛋白的表达水平，分析TLR9蛋白表达水平与相关临床指标间的相关性。结果 SLE患者T、B淋巴细胞中表达TLR9蛋白的细胞百分比分别为（53.94 ± 17.95）%、（49.33 ± 23.30）%，对照组分别为（29.40 ± 10.54）%、（29.18 ± 14.78）%，两组相比差异有统计学意义（t值分别为6.11，3.73，P值均 < 0.01）。表达TLR9蛋白的B细胞百分比与SLEDAI成负相关（r = -0.39，P = 0.02），与IgA表达量成正相关（r = 0.74，P < 0.01）。结论 初发未治型SLE患者TLR9蛋白表达水平较对照组升高，表达TLR9蛋白的B细胞百分比与SLEDAI成负相关，与IgA表达量成正相关。

关键词: 红斑狼疮，系统性;T细胞、B细胞，Toll样受体9

Abstract:

Objective To study the expressions of Toll-like receptor 9 protein （TLR9） in peripheral B and T lymphocytes in newly diagnosed, untreated patients with systemic lupus erythematosus （SLE） and their relationship with clinical parameters. Methods Blood samples were obtained from 35 newly diagnosed, untreated patients with SLE and 16 healthy human controls. B, T lymphocytes and TLR9 protein were labeled with fluorescent antibodies, and the expressions of TLR9 protein were detected by flow cytometry in peripheral B and T lymphocytes. The relationship between TLR9 expression and clinical parameters was assessed. Results The proportions of B and T lymphocytes expressing TLR9 in newly diagnosed, untreated patients were （53.94 ± 17.95）% and （49.33 ± 23.30）%, respectively, compared to （29.40 ± 10.54）% and （29.18 ± 14.78）%, respectively, in healthy controls （t = 6.11，3.73, respectively, both P < 0.01）. Additionally, the proportion of B lymphocytes expressing TLR9 correlated negatively with SLE disease activity index （SLEDAI）（r = -0.39, P < 0.05）, but positively with the level of serum IgA antibody （r = 0.74, P < 0.01）. Conclusions The expression of TLR9 is elevated in peripheral T and B lymphocytes from patients with newly diagnosed, untreated SLE, and the proportion of TLR9-expressing B lymphocytes negatively correlates with SLEDAI, but positively correlates with the serum level of IgA antibody.