Abstract:

Objective: To investigate the quality status of serologic detection for Treponema pallidum (TP) in clinical laboratories of Shanghai. Methods A panel consisting of fives serologic samples including negative, lower-titer positive and higher-titer positive samples were distributed for each laboratory. The participants were required to detect these samples used them examine system and return the data. All date were entered the computer and statistical analyzed with SPSS 12.1, and to compared the results of detection with the target value of the samples. Results The rate of feedback from total 212 participant laboratories was up to 99%. The qualification ratio was 90.6% in treponema test, and 72.8% in nontreponema test, respectively. The eligible laboratory of both test for all these samples were below 30%. Among screening tests, the qualification ratios in higher titer were above those in lower titer. The use ratio of mechanical horizontal rotators in screening tests was only 28% on spot checks. conclusion　There are some problem in detecting weakly positive sample of screening tests in clinical laboratories of Shanghai. The results of nontreponema testing in weakly positive sample were the key factor to impact the quality status, and had significant difference in different grades hospitals. This means that it is necessary to improve the detection quality of the laboratories.