中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (5): 336-338.

• 论著 • 上一篇    下一篇

亚临床皮肤刺激后三种生理参数的变化

吴琰瑜1,王学民1,秦鸥2,谭益妹1,程英2,刘娜3   

  1. 1. 上海市皮肤病性病医院
    2.
    3. 上海市皮肤病性病医院皮肤与化妆品研究室
  • 收稿日期:2008-04-25 修回日期:2008-07-21 出版日期:2009-05-15 发布日期:2009-05-13
  • 通讯作者: 吴琰瑜

Changes of three physiological parameters after subclinical skin irritation

  • Received:2008-04-25 Revised:2008-07-21 Online:2009-05-15 Published:2009-05-13

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摘要：

目的　探讨经表皮失水（TEWL）、皮肤电容量（CAP）、皮肤表面pH值参数在亚临床刺激性皮炎诊断中应用的可能性。方法　通过1%十二烷基硫酸钠（SLS）开放性斑贴、10次胶带粘贴及0.75最小红斑量UVB照射三种不同的刺激方法，对30名健康女性受试者的前臂屈侧进行处理，共5 d。每天刺激之前进行临床评估。试验第0，6，7，8天进行TEWL、CAP及皮肤表面pH值检测。结果　所有受试者8天内临床评分均为0分；SLS刺激后第6，7，8天TEWL值分别为3.17 ± 3.07（g/m2h）、3.32 ± 2.84（g/m2h）、3.22 ± 2.36（g/m2h），较基础值0.40 ± 1.35（g/m2h）升高，差异均有统计学意义（均为P = 0.00）。SLS刺激后第6，7，8天CAP值较基础值下降, 皮肤表面pH值较基础值升高，与基础值间差异均有统计学意义（P ＜ 0.05）。胶带粘贴后第6，7，8天TEWL值分别为2.54 ± 1.85（g/m2h）、2.40 ± 2.16（g/m2h）、2.17 ± 1.99（g/m2h），较基础值0.11 ± 1.10（g/m2h）升高，差异均有统计学意义（均为P = 0.00）；胶带粘贴后第8天，皮肤表面pH值较基础值升高，差异有统计学意义（P = 0.00）；CAP无明显变化。0.75 MED UVB照射后各生物物理参数均无明显变化。结论　三种生物物理参数可以被用于亚临床性刺激性接触性皮炎的检测。不同参数对不同的刺激方法检测能力不同。

Abstract:

Objective To evaluate the diagnostic significance of transepidermal water loss （TEWL）, skin capacitance （CAP） and skin surface pH in subclinical irritant dermatitis. Methods Thirty healthy females took part in the 8-day study. Four areas were delineated on the flexor side of both forearms of each subject: one area received no irritation and served as the control, one was challenged by 1% sodium lauryl sulphate （SLS）, one by repeated tape stripping, and one by irradiation with 0.75 MED UVB. Irritations were continuously given for 5 days. Clinical evaluation was performed everyday before irritation. TEWL, CAP and skin surface pH were measured at baseline, on day 6, 7 and 8 after the first irritation. Results Clinical score maintained at 0 for all subjects through the 8-day study. After SLS irritation, TEWL was 3.17 ± 3.07 g/m2h on day 6, 3.32 ± 2.84 g/m2h on day 7 and 3.22 ± 2.36 g/m2h on day 8, and all were significantly higher than that on day 0 （0.40 ± 1.35 g/m2h, P < 0.01）. Similarly, increased skin surface pH was observed on day 6, 7 and 8 after SLS irritation compared with that at baseline （all P < 0.05）. On the contrast, CAP decreased on day 6, 7 and 8 after SLS irritation （all P < 0.05）. After tape-stripping, a significant increase was observed in TEWL on day 6, 7 and 8 compared with that at baseline （2.54 ± 1.85 g/m2h, 2.40 ± 2.16 g/m2h and 2.17 ± 1.99 g/m2h vs 0.11 ± 1.10 g/m2h, all P < 0.01）; significant increase of pH was noted only on day 8; meanwhile, there was no any significant change in CAP. UVB irradiation induced no obvious changes in any of these physiological parameters. Conclusions These three physiological parameters can be applied to the diagnosis of subclinical irritant contact dermatitis, and their diagnostic value varies with the pattern of irritation.