中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (4): 244-247.

• 论著 • 上一篇    下一篇

基质金属蛋白酶在曝光及非曝光部位皮肤中的表达

康玉英,鞠梅,顾恒,陈昆   

  1. 1. 中国医学科学院北京协和医学院皮肤病研究所
  • 收稿日期:2008-10-30 修回日期:2008-12-10 出版日期:2009-04-15 发布日期:2009-04-14
  • 通讯作者: 陈昆，kunchen181@yahoo.cn
  • 基金资助:

    中国医学科学院皮肤病研究所基金资助项目（IMBF20070602）;不符合上述请自行填写具体基金资助项目名称（编号）

Expression of matrix metalloproteinases in sun-exposed and -unexposed skin

  • Received:2008-10-30 Revised:2008-12-10 Online:2009-04-15 Published:2009-04-14

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摘要：

目的 检测基质金属蛋白酶－1、3、9（MMP-1、3、9）在曝光部位和非曝光部位皮肤中的表达，探讨它们在皮肤光老化机制中的作用。方法 应用免疫组化方法分别对23例女性健康志愿者前臂伸侧（曝光）和上臂内侧（非曝光部位）皮肤石蜡标本中的MMP-1、MMP-3、MMP-9进行检测，表达强度以免疫反应强度分布指数（IRIDI）表示，采用Wilcoxon符号秩和检验、Mann-Whitney秩和检验及Spearman秩相关分析进行统计学处理。结果 MMP-1、MMP-3、MMP-9在曝光和非曝光部位均表达，曝光部位皮肤组织中MMP-1、MMP-3、MMP-9的IRIDI均值（范围）分别为7.70（3 ～ 12）、9.22（6 ～ 12）、8.30（6 ～ 12），非曝光部位分别为4.26（2 ～ 6）、5.39（2 ～ 9）、4.04（1 ～ 6），曝光部位MMP-1、MMP-3、MMP-9的IRIDI均显著高于非曝光部位（均P < 0.01）。50岁以上组曝光部位皮肤组织MMP-1、MMP-3、MMP-9的IRIDI分别为9.17（6 ～ 12）、10.58（8 ～ 12）、8.92（8 ～ 12），非曝光部位分别为4.75（2 ～ 6）、6.42（4 ～ 9）、4.33（3 ～ 6），曝光部位均显著高于非曝光部位（P < 0.05）；50岁以下组曝光部位皮肤组织MMP-1、MMP-3、MMP-9的IRIDI分别为6.09（3 ～ 8）、7.73（6 ～ 9）、7.64（6 ～ 12），非曝光部位分别为3.73（2 ～ 6）、4.27（2 ～ 8）、3.73（1 ～ 6），曝光部位均显著高于非曝光部位（P < 0.05）；50岁以上组曝光部位显著高于50岁以下组，而两组非曝光部位皮肤组织MMP-1、MMP-9的IRIDI值差异无统计学意义（P > 0.05）。曝光部位皮肤组织MMP-1、MMP-3、MMP-9的IRIDI值与年龄呈正相关（r = 0.66、0.69、0.74，P < 0.01），非曝光部位皮肤组织MMP-1、MMP-9的IRIDI值与年龄无相关性。结论 MMP-1、MMP-3、MMP-9在曝光部位表达显著高于非曝光部位，三者在光老化的发生发展中均起作用，但作用机制可能不同。

关键词: 光老化;基质金属蛋白酶;曝光部位

Abstract:

Objective To study the expression of matrix metalloproteinase（MMP）-1, -3 and -9 in sun-exposed and -unexposed skin as well as its significance in the mechanism of skin photoaging. Methods Skin samples were resected from the extensor side of forearm （sun-exposed area） and flexor side of upper arm （sun-unexposed area） of 23 healthy female volunteers. The expression of MMP-1, -3 and -9 was detected by immunohistochemical staining in 46 skin samples. Immunoreactive intensity distribution index （IRIDI） was calculated to assess the expression of MMP-1, -3 and -9. Wilcoxon signed ranks test, Mann-Whitney U-test and Spearman rank correlation analysis were performed. Results MMP-1, -3 and -9 were expressed in both sun-exposed and -unexposed skin. The average IRIDI value for MMP-1, -3 and -9 was 7.70 （range, 3 to 12）, 9.22 （range, 6 to 12）, 8.30 （range, 6 to 12） in sun-exposed skin, and 4.26 （range, 2 to 6）, 5.39（range, 2 to 9）, 4.04（range, 1 to 6） in sun-unexposed skin, respectively; significant difference existed between sun-exposed and -unexposed skin in the three parameters （all P < 0.01）. A significant increase was observed in the average IRIDI value for MMP-1, -3 and -9 in sun-exposed skin vs. sun-unexposed skin in women above 50 years of age （9.17 vs 4.75, 10.58 vs 6.42, 8.92 vs 4.33, respectively, all P < 0.05）. In women younger than 50 years, the average IRIDI value for MMP-1, -3 and -9 was 6.09 （range, 3 to 8）, 7.73 （range, 6 to 9）, 7.64 （range, 6 to 12） in sun-exposed skin, significantly higher than that in sun-unexposed skin ［3.73 （range, 2 to 6）, 4.27 （range, 2 to 8）, 3.73 （range, 1 to 6）, all P < 0.05］. Increased IRIDI scores of MMP-1, -3 and -9 were noticed in sun-exposed skin in women above 50 years of age vs. those younger than 50 years, but there was no statistical difference in MMP-1 or MMP-9 between the two aged groups in sun-unexposed skin（all P > 0.05）. The IRIDI scores of MMP-1, MMP-3 and MMP-9 were positively correlated with age（r = 0.66, 0.69, 0.74, all P < 0.01） in sun-exposed skin, but the IRIDI scores of MMP-1 and MMP-9 uncorrelated with age in sun-unexposed skin. Conclusions There is an elevated expression of MMP-1, -3 and -9 in sun-exposed skin vs. sun-unexposed skin, hinting that these three MMPs play a role in the occurrence and development of photoaging, but their biological mechanism may be different.